Product Pathways - Nuclear Receptor Signaling
SRC-3 (11B1) Mouse mAb #2115
|W IF-IC F||H||Endogenous||160||Mouse IgG1|
Reactivity Key: H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
SRC-3 (11B1) Mouse mAb detects endogenous levels of total SRC-3 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant human SRC-3 polypeptide fragment (a.a. 1-250).
Western blot analysis of cell extracts from various cell lines, using SRC-3 (11B1) Mouse mAb.
Flow cytometric analysis of MCF-7 cells, using SRC-3 (11B1) Mouse mAb (blue) compared to a nonspecific negative control antibody (red).
Confocal immunofluorescence images of MCF-7 cells labeled with SRC-3 (11B1) Mouse mAb (red). Actin filaments have been labeled with fluorescein phalloidin. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
There are three members of the steroid receptor co-activator (SRC) family of proteins: SRC-1 (NCoA-1), SRC-2 (TIF2/GRIP1/NCoA-2), and SRC-3 (ACTR/pCIP/RAC3/TRAM-1/AIB1). All SRC family members share significant structural homology and function to stimulate transcription mediated by nuclear hormone receptors and other transcriptional activators such as Stat3, NF-κB, E2F1, and p53 (1-4). Two SRC proteins, SRC-1 and SRC-3, function as histone acetyltransferases (5,6). In addition, all three family members can recruit other histone acetyltransferases (CBP/p300, PCAF) and histone methyltransferases (PRMT1, CARM1) to target promoters and cooperate to enhance expression of many genes (5-8). The SRC proteins play important roles in multiple physiological processes including cell proliferation, cell survival, somatic cell growth, mammary gland development, female reproductive function, and vasoprotection (9). SRC-1 and SRC-3 are conduits for kinase-mediated growth factor signaling to the estrogen receptor and other transcriptional activators. Seven SRC-1 phosphorylation sites and six SRC-3 phosphorylation sites have been identified, which are induced by steroids, cytokines, and growth factors and involve multiple kinase signaling pathways (9-11). Research has shown that all three SRC family members are associated with increased activity of nuclear receptors in breast, prostate, and ovarian carcinomas. According to the literature, SRC-3 is frequently amplified or overexpressed in a number of cancers (12), and SRC-1/PAX3 and SRC-2/MYST3 translocations are found associated with rhabdomyosarcoma and acute myeloid leukemia, respectively (13,14).
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- Wu, R.C. et al. (2004) Mol. Cell 15, 937-949.
- Rowan, B.G. et al. (2000) J. Biol. Chem. 275, 4475-4483.
- Zhou, H.J. et al. (2005) Cancer Res. 65, 7976-7983.
- Torres-Arzayus, M.I. et al. (2004) Cancer Cell 6, 263-274.
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For Research Use Only. Not For Use In Diagnostic Procedures.