Cell Signaling Technology

Product Pathways - Nuclear Receptor Signaling

SRC-3 (11B1) Mouse mAb #2115

Applications Reactivity Sensitivity MW (kDa) Isotype
W IF-IC F H Endogenous 160 Mouse IgG1

Applications Key:  W=Western Blotting  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

SRC-3 (11B1) Mouse mAb detects endogenous levels of total SRC-3 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant human SRC-3 polypeptide fragment (a.a. 1-250).

Western Blotting

Western Blotting

Western blot analysis of cell extracts from various cell lines, using SRC-3 (11B1) Mouse mAb.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of MCF-7 cells, using SRC-3 (11B1) Mouse mAb (blue) compared to a nonspecific negative control antibody (red).

IF-IC

IF-IC

Confocal immunofluorescence images of MCF-7 cells labeled with SRC-3 (11B1) Mouse mAb (red). Actin filaments have been labeled with fluorescein phalloidin. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).


Background

There are three members of the steroid receptor co-activator (SRC) family of proteins: SRC-1 (NCoA-1), SRC-2 (TIF2/GRIP1/NCoA-2), and SRC-3 (ACTR/pCIP/RAC3/TRAM-1/AIB1). All SRC family members share significant structural homology and function to stimulate transcription mediated by nuclear hormone receptors and other transcriptional activators such as Stat3, NF-κB, E2F1, and p53 (1-4). Two SRC proteins, SRC-1 and SRC-3, function as histone acetyltransferases (5,6). In addition, all three family members can recruit other histone acetyltransferases (CBP/p300, PCAF) and histone methyltransferases (PRMT1, CARM1) to target promoters and cooperate to enhance expression of many genes (5-8). The SRC proteins play important roles in multiple physiological processes including cell proliferation, cell survival, somatic cell growth, mammary gland development, female reproductive function, and vasoprotection (9). SRC-1 and SRC-3 are conduits for kinase-mediated growth factor signaling to the estrogen receptor and other transcriptional activators. Seven SRC-1 phosphorylation sites and six SRC-3 phosphorylation sites have been identified, which are induced by steroids, cytokines, and growth factors and involve multiple kinase signaling pathways (9-11). Research has shown that all three SRC family members are associated with increased activity of nuclear receptors in breast, prostate, and ovarian carcinomas. According to the literature, SRC-3 is frequently amplified or overexpressed in a number of cancers (12), and SRC-1/PAX3 and SRC-2/MYST3 translocations are found associated with rhabdomyosarcoma and acute myeloid leukemia, respectively (13,14).

  1. Giraud, S. et al. (2002) J. Biol. Chem. 277, 8004-8011.
  2. Na, S.Y. et al. (1998) J. Biol. Chem. 273, 10831-10834.
  3. Louie, M.C. et al. (2004) Mol. Cell Biol. 24, 5157-5171.
  4. Lee, S.K. et al. (1999) Mol. Endocrinol. 13, 1924-1933.
  5. Spencer, T.E. et al. (1997) Nature 389, 194-198.
  6. Chen, H. et al. (1997) Cell 90, 569-580.
  7. Koh, S.S. et al. (2001) J. Biol. Chem. 276, 1089-1098.
  8. Chen, D. et al. (1999) Science 284, 2174-2177.
  9. Wu, R.C. et al. (2004) Mol. Cell 15, 937-949.
  10. Rowan, B.G. et al. (2000) J. Biol. Chem. 275, 4475-4483.
  11. Zhou, H.J. et al. (2005) Cancer Res. 65, 7976-7983.
  12. Torres-Arzayus, M.I. et al. (2004) Cancer Cell 6, 263-274.
  13. Wachtel, M. et al. (2004) Cancer Res. 64, 5539-5545.
  14. Deguchi, K. et al. (2003) Cancer Cell 3, 259-271.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Products