Product Pathways - Cytoskeletal Signaling
β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate) #2116
| Applications | Reactivity | Sensitivity | Isotype |
|---|---|---|---|
| IF-IC | H M R Mk | Endogenous | Rabbit IgG |
Applications Key:
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate) detects endogenous levels of total β-tubulin protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide (KLH-coupled) corresponding to the amino terminus of human β-tubulin. The antibody was conjugated to Alexa Fluor® 555 under optimal conditions with an F/P ratio of 2-6.
IF-IC
Confocal immunofluorescent analysis of HeLa cells, serum-starved (left) or 20% serum-treated (right), using β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate) (red) and Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb (Alexa Fluor® 488 Conjugate) #4854 (green). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
Description
Cell Signaling Technology antibody is conjugated to Alexa Fluor® 555 fluorescent dye and tested in-house for direct immunofluorescent analysis of human and monkey cells. The unconjugated antibody #2128 reacts with human, mouse, rat and monkey β-tubulin protein. CST expects that β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate) will also recognize β-tubulin in these species.
Background
The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is necessary to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1).
Application References
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Companion Products
- 2144 α-Tubulin Antibody
- 2125 α-Tubulin (11H10) Rabbit mAb
- 2146 β-Tubulin Antibody
- 2148 α/β-Tubulin Antibody
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
