Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

RhoA (67B9) Rabbit mAb #2117

Applications Reactivity Sensitivity MW (kDa) Isotype
W H M R Mk B Endogenous 21 Rabbit IgG

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

RhoA (67B9) Rabbit mAb recognizes endogenous levels of total RhoA protein. The antibody does not recognize the related proteins RhoB or RhoC.

Source / Purification

Monoclonal antibody is produced by immunizing animals with synthetic peptides corresponding to residues near the carboxy terminus of human RhoA.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines, using RhoA (67B9) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of recombinant GST-tagged RhoA, RhoB and RhoC using RhoA (67B9) Rabbit mAb.

Background

Rho family small GTPases, including Rho, Rac and cdc42, act as molecular switches, regulating processes such as cell migration, adhesion, proliferation and differentiation. They are activated by guanine nucleotide exchange factors (GEFs), which catalyze the exchange of bound GDP for GTP, and inhibited by GTPase activating proteins (GAPs), which catalyze the hydrolysis of GTP to GDP. A third level of regulation is provided by the stoichiometric binding of Rho GDP dissociation inhibitor (RhoGDI) (1). RhoA, RhoB and RhoC are highly homologous, but appear to have divergent biological functions. Carboxy-terminal modifications and differences in subcellular localization allow these three proteins to respond to and act on distinct signaling molecules (2,3).

Functions of RhoA, the most highly studied of these three, include regulation of actomyosin contractility (4), cytokinesis (5), focal adhesion assembly (6) and cell polarity (7).

  1. DerMardirossian, C. and Bokoch, G.M. (2005) Trends Cell Biol 15, 356-63.
  2. Wennerberg, K. and Der, C.J. (2004) J Cell Sci 117, 1301-12.
  3. Wheeler, A.P. and Ridley, A.J. (2004) Exp Cell Res 301, 43-9.
  4. Bi, D. et al. (2005) Circ Res 96, 890-7.
  5. Kimura, K. et al. (2000) J Biol Chem 275, 17233-6.
  6. Barry, S.T. and Critchley, D.R. (1994) J Cell Sci 107 ( Pt 7), 2033-45.
  7. Van Keymeulen, A. et al. (2006) J Cell Biol 174, 437-45.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

Products