Product Pathways - Cytoskeletal Signaling

RhoA (67B9) Rabbit mAb #2117

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Applications	Reactivity	MW (kDa)	Source	Isotype
W	H M R Mk B	21	Rabbit	IgG

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  B=Bovine
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

RhoA (67B9) Rabbit mAb recognizes endogenous levels of total RhoA protein. The antibody does not recognize the related proteins RhoB or RhoC.

Source / Purification

Monoclonal antibody is produced by immunizing rabbits with synthetic peptides (KLH-coupled) corresponding to residues near the carboxy terminus of human RhoA.

Background

Rho family small GTPases, including Rho, Rac and cdc42, act as molecular switches, regulating processes such as cell migration, adhesion, proliferation and differentiation. They are activated by guanine nucleotide exchange factors (GEFs), which catalyze the exchange of bound GDP for GTP, and inhibited by GTPase activating proteins (GAPs), which catalyze the hydrolysis of GTP to GDP. A third level of regulation is provided by the stoichiometric binding of Rho GDP dissociation inhibitor (RhoGDI) (1). RhoA, RhoB and RhoC are highly homologous, but appear to have divergent biological functions. Carboxy-terminal modifications and differences in subcellular localization allow these three proteins to respond to and act on distinct signaling molecules (2,3).

Functions of RhoA, the most highly studied of these three, include regulation of actomyosin contractility (4), cytokinesis (5), focal adhesion assembly (6) and cell polarity (7).

1. DerMardirossian, C. and Bokoch, G.M. (2005) Trends Cell Biol 15, 356-63.
2. Wennerberg, K. and Der, C.J. (2004) J Cell Sci 117, 1301-12.
3. Wheeler, A.P. and Ridley, A.J. (2004) Exp Cell Res 301, 43-9.
4. Bi, D. et al. (2005) Circ Res 96, 890-7.
5. Kimura, K. et al. (2000) J Biol Chem 275, 17233-6.
6. Barry, S.T. and Critchley, D.R. (1994) J Cell Sci 107 ( Pt 7), 2033-45.
7. Van Keymeulen, A. et al. (2006) J Cell Biol 174, 437-45.

Application References

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Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.