Product Pathways - Cytoskeletal Signaling
RhoA (67B9) Rabbit mAb #2117
|2117S||100 µl (10 western blots)||---||In Stock||---|
|2117P||40 µl (4 western blots)||---||In Stock||---|
|2117||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat, Monkey, Bovine||Endogenous||21||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Specificity / Sensitivity
RhoA (67B9) Rabbit mAb recognizes endogenous levels of total RhoA protein. The antibody does not recognize the related proteins RhoB or RhoC.
Source / Purification
Monoclonal antibody is produced by immunizing animals with synthetic peptides corresponding to residues near the carboxy terminus of human RhoA.
Western blot analysis of extracts from various cell lines, using RhoA (67B9) Rabbit mAb.
Rho family small GTPases, including Rho, Rac and cdc42, act as molecular switches, regulating processes such as cell migration, adhesion, proliferation and differentiation. They are activated by guanine nucleotide exchange factors (GEFs), which catalyze the exchange of bound GDP for GTP, and inhibited by GTPase activating proteins (GAPs), which catalyze the hydrolysis of GTP to GDP. A third level of regulation is provided by the stoichiometric binding of Rho GDP dissociation inhibitor (RhoGDI) (1). RhoA, RhoB and RhoC are highly homologous, but appear to have divergent biological functions. Carboxy-terminal modifications and differences in subcellular localization allow these three proteins to respond to and act on distinct signaling molecules (2,3).
Functions of RhoA, the most highly studied of these three, include regulation of actomyosin contractility (4), cytokinesis (5), focal adhesion assembly (6) and cell polarity (7).
- DerMardirossian, C. and Bokoch, G.M. (2005) Trends Cell Biol 15, 356-63.
- Wennerberg, K. and Der, C.J. (2004) J Cell Sci 117, 1301-12.
- Wheeler, A.P. and Ridley, A.J. (2004) Exp Cell Res 301, 43-9.
- Bi, D. et al. (2005) Circ Res 96, 890-7.
- Kimura, K. et al. (2000) J Biol Chem 275, 17233-6.
- Barry, S.T. and Critchley, D.R. (1994) J Cell Sci 107 ( Pt 7), 2033-45.
- Van Keymeulen, A. et al. (2006) J Cell Biol 174, 437-45.
- Kamanova, J. et al. (2008) J Immunol 181, 5587-97. Applications: Western Blotting.
- Zaytseva, Y.Y. et al. (2012) Cancer Res 72, 1504-17. Applications: Western Blotting.
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
This antibody is developed, validated, and produced by CST using in part technology under license (granting certain rights including those under U.S. Patents No. 5,675,063 and 7,429,487) from Epitomics, Inc.