Product Pathways - Glucose Metabolism
GAPDH (14C10) Rabbit mAb #2118
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IHC-P IF-IC F | H M R Mk | Endogenous | 37 | Rabbit IgG |
Applications Key:
W=Western Blotting
IHC-P=Immunohistochemistry (Paraffin)
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
GAPDH (14C10) Rabbit mAb detects endogenous levels of total GAPDH protein.
Source / Purification
Monoclonal antibody is produced by immunizing rabbits with synthetic peptides (KLH-coupled) corresponding to the sequence of human GAPDH.
Western Blotting
Western blot analysis of extracts from various cell lines using GAPDH (14C10) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using GAPDH (14C10) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using GAPDH (14C10) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
Flow Cytometry
Flow cytometric analysis of HeLa cells using GAPDH (14C10) Rabbit mAb antibody (blue) compared to a nonspecific negative control antibody (red).
IF-IC
Confocal immunofluorescent analysis of HeLa cells using GAPDH (14C10) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
Background
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. Though differentially expressed from tissue to tissue (1), GAPDH is thought to be a constitutively expressed housekeeping protein. For this reason, GAPDH mRNA and protein levels are often measured as controls in experiments quantifying specific changes in expression of other targets. Recent work has elucidated roles for GAPDH in apoptosis (2), gene expression (3) and nuclear transport (4). GAPDH may also play a role in neurodegenerative pathologies such as Huntington's and Alzheimer's disease (4,5).
- Barber, R.D. et al. (2005) Physiol. Genomics 21, 389-95.
- Hara, M.R. and Snyder, S.H. (2006) Cell Mol. Neurobiol. , Eput ahead of print.
- Zheng, L. et al. (2003) Cell 114, 255-66.
- Bae, B.I. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 3405-9.
- Wang, Q. et al. (2005) FASEB J. 19, 869-71.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
