Product Pathways - Metabolism
GAPDH (14C10) Rabbit mAb #2118
Have you tried your application using our XP® monoclonal antibodies? Try product: 5174
PhosphoSitePlus® protein, site, and accession data: GAPDH
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IHC-P IF-IC F | H M R Mk (Pg) | Endogenous | 37 | Rabbit IgG |
Applications Key:
W=Western Blotting
IHC-P=Immunohistochemistry (Paraffin)
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Pg=Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
GAPDH (14C10) Rabbit mAb detects endogenous levels of total GAPDH protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide near the carboxy terminus of human GAPDH.
Western Blotting
Western blot analysis of extracts from various cell lines using GAPDH (14C10) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using GAPDH (14C10) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using GAPDH (14C10) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
Flow Cytometry
Flow cytometric analysis of HeLa cells using GAPDH (14C10) Rabbit mAb antibody (blue) compared to a nonspecific negative control antibody (red).
IF-IC
Confocal immunofluorescent analysis of HeLa cells using GAPDH (14C10) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Background
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. Though differentially expressed from tissue to tissue (1), GAPDH is thought to be a constitutively expressed housekeeping protein. For this reason, GAPDH mRNA and protein levels are often measured as controls in experiments quantifying specific changes in expression of other targets. Recent work has elucidated roles for GAPDH in apoptosis (2), gene expression (3), and nuclear transport (4). GAPDH may also play a role in neurodegenerative pathologies such as Huntington and Alzheimer's diseases (4,5).
- Barber, R.D. et al. (2005) Physiol. Genomics 21, 389-95.
- Hara, M.R. and Snyder, S.H. (2006) Cell Mol. Neurobiol. 26, 527-38.
- Zheng, L. et al. (2003) Cell 114, 255-66.
- Bae, B.I. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 3405-9.
- Wang, Q. et al. (2005) FASEB J. 19, 869-71.
Application References
- Bjorklund, N.L. et al. (2012) J Neurosci Methods 203, 146-51. Applications: Western Blotting
- Carlin, L.M. et al. (2011) Sci Signal 4, ra81. Applications: Western Blotting
- Siemens, N. et al. (2011) J Biol Chem 286, 21612-22. Applications: Western Blotting
- Wu, M. et al. (2010) Rejuvenation Res 13, 571-9. Applications: Western Blotting
- Matheny, R.W. and Nindl, B.C. (2011) Endocrinology 152, 1923-34. Applications: Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
For Research Use Only. Not For Use In Diagnostic Procedures.
