Product Pathways - Metabolism
GAPDH (14C10) Rabbit mAb #2118
|2118L||300 µl (30 western blots)||---||In Stock||---|
|2118S||100 µl (10 western blots)||---||In Stock||---|
|2118||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat, Monkey, Bovine||Endogenous||37||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Species predicted to react based on 100% sequence homology: Pig.
Specificity / Sensitivity
GAPDH (14C10) Rabbit mAb detects endogenous levels of total GAPDH protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide near the carboxy terminus of human GAPDH.
Western blot analysis of extracts from various cell lines using GAPDH (14C10) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using GAPDH (14C10) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using GAPDH (14C10) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
Flow cytometric analysis of HeLa cells using GAPDH (14C10) Rabbit mAb antibody (blue) compared to a nonspecific negative control antibody (red).
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. Though differentially expressed from tissue to tissue (1), GAPDH is thought to be a constitutively expressed housekeeping protein. For this reason, GAPDH mRNA and protein levels are often measured as controls in experiments quantifying specific changes in expression of other targets. Recent work has elucidated roles for GAPDH in apoptosis (2), gene expression (3), and nuclear transport (4). GAPDH may also play a role in neurodegenerative pathologies such as Huntington and Alzheimer's diseases (4,5).
- Israelsen, W.J. et al. (2013) Cell 155, 397-409. Applications: Western Blotting.
- Bjorklund, N.L. et al. (2012) J Neurosci Methods 203, 146-51. Applications: Western Blotting.
- Matheny, R.W. and Nindl, B.C. (2011) Endocrinology 152, 1923-34. Applications: Western Blotting.
- Carlin, L.M. et al. (2011) Sci Signal 4, ra81. Applications: Western Blotting.
- Siemens, N. et al. (2011) J Biol Chem 286, 21612-22. Applications: Western Blotting.
- Wu, M. et al. (2010) Rejuvenation Res 13, 571-9. Applications: Western Blotting.
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For Research Use Only. Not For Use In Diagnostic Procedures.
DRAQ5® is a registered trademark of Biostatus Limited.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
This antibody is developed, validated, and produced by CST using in part technology under license (granting certain rights including those under U.S. Patents No. 5,675,063 and 7,429,487) from Epitomics, Inc.