Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Src (32G6) Rabbit mAb #2123

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M R (C) Endogenous 60 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  C=Chicken
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Src (32G6) Rabbit mAb detects endogenous levels of total Src proteins. The antibody does not cross-react with other proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant fusion protein corresponding to residues 1-110 of human Src protein.

Western Blotting

Western Blotting

Western blot analysis of cell extracts from various cell lines, using Src (32G6) Rabbit mAb.

Background

The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2).

  1. Thomas, S.M. and Brugge, J.S. (1997) Annu. Rev. Cell Dev. Biol. 13, 513-609.
  2. Hunter, T. (1987) Cell 49, 1-4.

Application References

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Companion Products

Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.

For Research Use Only. Not For Use In Diagnostic Procedures.

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