Product Pathways - Tyrosine Kinase/ Adaptors
Src (32G6) Rabbit mAb #2123
| Applications | Reactivity | Sensitivity | MW (kDa) | Source | Isotype |
|---|---|---|---|---|---|
| W IP IF-IC F | H M R (C) | Endogenous | 60 | Rabbit | IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
R=Rat
C=Chicken
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Src (32G6) Rabbit mAb detects endogenous levels of total Src proteins. The antibody does not cross-react with other proteins.
Source / Purification
Monoclonal antibodies are produced by immunizing rabbits with a GST-Src fusion protein containing the corresponding amino acid residues 1-110 of human Src.
Western Blotting
Western blot analysis of cell extracts from various cell lines, using Src (32G6) Rabbit mAb.
Flow Cytometry
Flow cytometric analysis of Jurkat cells, using Src (32G6) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).
IF-F
Confocal immunofluorescent analysis of MCF7 cells labeled with Src (32G6) Rabbit mAb (red). Blue=DRAQ5™ fluorescent DNA dye.
Background
The Src family of protein tyrosine kinases (including Src, Lyn, Fyn, Yes, Lck, Blk and Hck) are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. Phosphorylation of Tyr416 in the activation loop of the kinase domain by Csk upregulates enzyme activity, whereas phosphorylation of Tyr527 in the carboxy-terminal tail renders the enzyme less active (2).
- Thomas, S.M. and Brugge, J.S. (1997) Annu. Rev. Cell Dev. Biol. 13, 513-609.
- Hunter, T. (1987) Cell 49, 1-4.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
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Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
