Cell Signaling Technology

Product Pathways - Tyrosine Kinase/ Adaptors

Src (32G6) Rabbit mAb #2123

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M R (C) Endogenous 60 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  C=Chicken
Species cross-reactivity is determined by Western blot.

Protocols

Specificity / Sensitivity

Src (32G6) Rabbit mAb detects endogenous levels of total Src proteins. The antibody does not cross-react with other proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a GST-Src fusion protein containing the corresponding amino acid residues 1-110 of human Src.

Western Blotting

Western Blotting

Western blot analysis of cell extracts from various cell lines, using Src (32G6) Rabbit mAb.

Background

The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk and Hck, are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. Phosphorylation of Src at Tyr416 in the activation loop of the kinase domain by carboxy-terminal Src kinase (Csk) upregulates enzyme activity, whereas phosphorylation of Tyr527 in the carboxy-terminal tail renders the enzyme less active (2).

  1. Thomas, S.M. and Brugge, J.S. (1997) Annu. Rev. Cell Dev. Biol. 13, 513-609.
  2. Hunter, T. (1987) Cell 49, 1-4.

Application References

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Companion Products

Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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