Product Pathways - Cytoskeletal Signaling
α-Tubulin (11H10) Rabbit mAb #2125
PhosphoSitePlus® protein, site, and accession data: TUBA1B
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IHC-P IF-IC F | H M R Mk Dm B (Dg) | Endogenous | 52 | Rabbit IgG |
Applications Key:
W=Western Blotting
IHC-P=Immunohistochemistry (Paraffin)
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Dm=D. melanogaster
B=Bovine
Dg=Dog
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
α-Tubulin (11H10) Rabbit mAb detects endogenous levels of total α-tubulin protein, and does not cross-react with recombinant β-tubulin.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of human α-tubulin protein.
Western Blotting
Western blot analysis of extracts from C6, COS-7, NIH/3T3 and HeLa cells, using α-Tubulin (11H10) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human glioblastoma, using α-Tubulin (11H10) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human melanoma, using α-Tubulin (11H10) Rabbit mAb.
Flow Cytometry
Flow cytometric analysis of HeLa cells, using α-Tubulin (11H10) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).
IF-IC
Confocal immunofluorescent analysis of HeLa cells, using α-Tubulin (11H10) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Background
The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1).
Application References
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Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
