Product Pathways - Cytoskeletal Signaling
β-Tubulin (9F3) Rabbit mAb #2128
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IHC-P IF-IC F | H M R Mk B | Endogenous | 55 | Rabbit IgG |
Applications Key:
W=Western Blotting
IHC-P=Immunohistochemistry (Paraffin)
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
B=Bovine
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
β-Tubulin (9F3) Rabbit mAb detects endogenous levels of total β-tubulin protein, and does not cross-react with recombinant α-tubulin.
Source / Purification
Monoclonal antibody is produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to the amino terminus of human β-tubulin.
Western Blotting
Western blot analysis of extracts from COS-7, NIH/3T3 and PC12 cells, using β-Tubulin (9F3) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human glioblastoma, using β-Tubulin (9F3) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using β-Tubulin (9F3) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human melanoma, using β-Tubulin (9F3) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using β-Tubulin (9F3) Rabbit mAb preincubated with control peptide (left) or β-Tubulin Blocking Peptide #1032 (right).
Flow Cytometry
Flow cytometric analysis of NIH/3T3 cells using β-Tubulin (9F3) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).
IF-IC
Confocal immunofluorescent analysis of HeLa cells, using β-Tubulin (9F3) Rabbit mAb (green). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
Background
The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is necessary to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1).
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
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- 2125 α-Tubulin (11H10) Rabbit mAb
- 2148 α/β-Tubulin Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 1032 β-Tubulin Blocking Peptide
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
