Product Pathways - Nuclear Receptor Signaling
Phospho-Rev-erbα (Ser55/59) Antibody #2129
|W||H (M) (R) (B)||Transfected Only||80||Rabbit|
Reactivity Key: H=Human M=Mouse R=Rat B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Phospho-Rev-erbα (Ser55/59) Antibody detects transfected levels of Rev-erbα protein when phosphorylated at Ser55/59. The antibody does not cross-react with other nuclear receptor proteins, including Rev-erbβ.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser55 and Ser59 of human Rev-erbα. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from 293 cells, either untransfected or transfected with DYKDDDDK-tagged Rev-erbα expression constructs, using Phospho-Rev-erbα (Ser55/Ser59) Antibody (upper) or DYKDDDDK Tag Antibody (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody) #2368 (lower). The phospho-specificity of the antibody was verified by transfecting cells with a mutant DYKDDDDK-Rev-erbα (Ser55/59Ala) expression construct and by incubating 293/Rev-erbα (WT) extracts with (+) or without (-) λ phosphatase.
Reverse orientation c-erbA gene α (Rev-erbα, EAR-1, or NR1D1) is a widely expressed member of the orphan nuclear receptor family of proteins (1). Rev-erbα is highly expressed in adipose tissue, skeletal muscle, brain and liver, and regulates cellular proliferation and differentiation. Expression increases during differentiation in adipocytes and ectopic expression of Rev-erbα potentiates the adipocyte differentiation of 3T3-L1 cells (2). In addition, expression oscillates with circadian rhythm in liver cells and Rev-erbα regulates expression of BMAL1, ApoA-I and ApoC-III, all key regulators of circadian rhythm (3,4,5,6,7). Phosphorylation of Rev-erbα Ser55 and Ser59 by GSK3β appears to stabilize Rev-erbα protein levels and is important for synchronizing and maintaining the circadian clock (8). Rev-erbα also regulates inflammation by targeting the NF-κB responsive genes IL-6 and COX-2 (9). Rev-erbα lacks the activation function 2 domain required for ligand-dependent activation of transcription by other members of the nuclear receptor family; thus it behaves as a constitutive repressor protein, recruiting the nuclear receptor co-repressor (N-CoR)/HDAC3 complex to target genes to repress transcription (10).
- Harding, H.P. and Lazar, M.A. (1993) Mol. Cell Biol. 13, 3113-3121.
- Chawla, A. and Lazar, M.A. (1993) J. Biol. Chem. 268, 16265-16269.
- Torra, I.P. et al. (2000) Endocrinology 141, 3799-3806.
- Preitner, N. et al. (2002) Cell 110, 251-260.
- Vu-Dac, N. et al. (1998) J. Biol .Chem. 273, 25713-25720.
- Coste, H. and Rodríguez, J.C. (2002) J. Biol. Chem. 277, 27120-27129.
- Raspé, E. et al. (2002) J. Lipid Res. 43, 2172-2179.
- Yin, L. et al. (2006) Science 311, 1002-1005.
- Migita, H. et al. (2004) FEBS Lett. 561, 69-74.
- Yin, L. and Lazar, M.A. (2005) Mol. Endocrinol. 19, 1452-1459.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 9336 Phospho-GSK-3β (Ser9) Antibody
- 9315 GSK-3β (27C10) Rabbit mAb
- 2632 Histone Deacetylase 3 (HDAC3) Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 4842 Cox2 Antibody
Anti-FLAG® is a registered trademark of Sigma-Aldrich.
For Research Use Only. Not For Use In Diagnostic Procedures.