Product Pathways - Neuroscience
Parkin Antibody #2132
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M R | Endogenous | 52 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Parkin Antibody detects endogenous levels of total parkin protein.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues surrounding amino acid 400 of human parkin. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from parkin-transfected SH-SY5Y cells (lane 1), untransfected SH-SY5Y cells (lane 2) and C6 cells (lane 3), using Parkin Antibody.
Background
Parkin is a protein of 465 amino acids with an amino-terminal ubiquitin domain and a carboxy-terminal RING-box (1). In the case of autosomal recessive juvenile Parkinsonism (AR-JP), deletions have been found in the gene on chromosome 6 encoding the protein Parkin (2).
- Tanner, C.M. and Goldman, S.M. (1996) Neurol. Clin. 14, 317-335.
- Polymeropoulous, M.H. et al. (1997) Science 276, 2045-2047.
Application References
- Cesari, , R. et al. (2003) Parkin, a gene implicated in autosomal recessive juvenile parkinsonism, is a candidate tumor suppressor gene on chromosome 6q25-q27. Proc Natl Acad Sci U S A 100, 5956-5961. This article references the use of Parkin Antibody in the following applications: Western Blotting
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Companion Products
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- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
