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Parkin Antibody #2132

PhosphoSitePlus^® protein, site, and accession data: PARK2

Applications	Reactivity	Sensitivity	MW (kDa)	Source
W	H M R	Endogenous	52	Rabbit

Applications Key: W=Western Blotting
Reactivity Key: H=Human M=Mouse R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

2132:: Western Blotting

Specificity / Sensitivity

Parkin Antibody detects endogenous levels of total parkin protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding amino acid 400 of human parkin. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western blot analysis of extracts from parkin-transfected SH-SY5Y cells (lane 1), untransfected SH-SY5Y cells (lane 2) and C6 cells (lane 3), using Parkin Antibody.

Background

Parkin is a protein of 465 amino acids with an amino-terminal ubiquitin domain and a carboxy-terminal RING-box (1). In the case of autosomal recessive juvenile Parkinsonism (AR-JP), deletions have been found in the gene on chromosome 6 encoding the protein Parkin (2).

Application References

Cesari, , R. et al. (2003) Parkin, a gene implicated in autosomal recessive juvenile parkinsonism, is a candidate tumor suppressor gene on chromosome 6q25-q27. Proc Natl Acad Sci U S A 100, 5956-5961. Applications: Western Blotting

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For Research Use Only. Not For Use In Diagnostic Procedures.