Cell Signaling Technology

Product Pathways - Neuroscience

DJ-1 Antibody #2134

Applications Reactivity Sensitivity MW (kDa) Source
W F H M R Endogenous 22 Rabbit

Applications Key:  W=Western Blotting  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

DJ-1 Antibody detects endogenous levels of total DJ-1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding amino acid 150 of human DJ-1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa and CAD cells, using DJ-1 Antibody.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Hela cells, using DJ-1 Antibody (blue) compared to a nonspecific negative control antibody (red).

Background

Parkinson's disease (PD) is characterized by the presence of Lewy bodies (intracellular inclusions) and by the loss of dopaminergic neurons. Research studies have shown that mutations in α-synuclein, Parkin, and DJ-1 are linked to PD (1). α-synuclein is a major component of the aggregates found in Lewy bodies. Parkin is involved in protein degradation through the ubiquitin-proteasome pathway, and investigators have shown that mutations in Parkin cause early onset of PD (1). Loss-of-function mutations in DJ-1 cause early onset of PD, but DJ-1 is associated with multiple functions: it cooperates with Ras to increase cell transformation, it positively regulates transcription of the androgen receptor, and it may function as an indicator of oxidative stress (2-5). Dopamine D2 receptor-mediated functions are greatly impaired in DJ-1 (-/-) mice, resulting in reduced long-term depression (6).

  1. Borrelli, E. (2005) Neuron 45, 479-81.
  2. Bonifati, V. et al. (2003) Science 299, 256-9.
  3. Nagakubo, D. et al. (1997) Biochem. Biophys. Res. Commun. 231, 509-13.
  4. Takahashi, K. et al. (2001) J. Biol. Chem. 276, 37556-63.
  5. Mitsumoto, A. and Nakagawa, Y. (2001) Free Radic. Res. 35, 885-93.
  6. Goldberg, M.S. et al. (2005) Neuron 45, 489-96.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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