Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

CD2AP Antibody #2135

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Mk Endogenous 80 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

CD2AP Antibody detects endogenous levels of total CD2AP protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids near the carboxy terminus of human CD2AP. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts of K562, A431 and COS cells, using CD2AP Antibody.

Background

CD2AP is a scaffolding protein that is thought to link membrane proteins to the cytoskeleton (1-3). It plays a role in formation of tight junctions in specialized cell types such as the slit diaphragm in the kidney glomerulus (4). CD2AP is also involved in the immunological synapse between CD2 expressing T cells and antigen presenting cells (5). It has been shown that interaction between CD2AP and other cytoskeletal proteins may regulate the endocytosis of EGFR (3).

  1. Kirsch, K.H. et al. (1999) Proc. Natl. Acad. Sci. USA 96, 6211-6216.
  2. Kirsch, K.H. et al. (2001) J. Biol. Chem. 276, 4957-4963.
  3. Lynch, D.K. et al. (2003) J. Biol. Chem. 278, 21805-21813.
  4. Kawachi, H. et al. (2006) Nephrology (Carlton) 11, 274-281.
  5. Hutchings, N.J. et al. (2003) J. Biol. Chem. 278, 22396-22403.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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