Cell Signaling Technology

Product Pathways - Neuroscience

Calbindin Antibody #2136

Applications Reactivity MW (kDa) Source
W IHC-P IF-F H M R 28 Rabbit

Applications Key:  W=Western Blotting  IHC-P=Immunohistochemistry (Paraffin)  IF-F=Immunofluorescence (Frozen)
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Calbindin Antibody detects endogenous levels of total calbindin protein.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues surrounding amino acid 200 of human calbindin. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from human cerebellum and rat brain, using Calbindin Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse brain, using Calbindin Antibody.

IF-F

IF-F

Confocal immunofluorescent image of mouse cerebellum labeled with Calbindin Antibody (green) and Neurofilament-L (DA2) Mouse mAb #2835 (red).


Background

Calcium-binding proteins of different subfamilies regulate the second messenger calcium. Calbindin, calmodulin, S-100, paravalbumin and troponin C are members of the low molecular weight calcium-binding protein family (1). Calbindin is expressed in discrete neuronal populations within the CNS and is thought to act as an intracellular calcium buffering protein. Most Purkinje cells express calbindin, which is expressed when neurons start to migrate and differentiate. In contrast, other calcium buffering proteins, such as parvalbumin, are expressed later during development and in parallel with increasing neuronal activity (2).

  1. Kawasaki, H. et al. (1998) Biometals 11, 277-95.
  2. Bastianelli, E. (2003) Cerebellum 2, 242-62.

Application References

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