Product Pathways - Wnt / Hedgehog / Notch
AFP Antibody #2137
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | H M | Endogenous | 70 kDa | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
Species cross-reactivity is determined by Western blot.
Protocols
Specificity / Sensitivity
AFP Antibody detects endogenous levels of total AFP protein in mouse and human cells. The antibody can only immunoprecipitate human AFP.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids near the carboxy terminus of human AFP. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of recombinant AFP, using AFP Antibody.
Background
Alpha-fetoprotein (AFP) is a 65 kDa glycoprotein found in the serum of the mammalian fetal liver, yolk sac and GI tract. Expression of AFP in adult cells is low. However, synthesis aberrantly occurs in adult liver cancer cells (1,2). The tumor suppressor gene p53 and β-catenin are both involved in the regulation of AFP expression. In normal adult cells p53 binds to the repressor region of the AFP gene, thereby blocking transcription. Mutations in both p53 and β-catenin are associated with aberrant expression of AFP. Elevated serum levels are predictive of hepatocellular carcinoma (3).
- Meier, V. et al. (2006) Comp Hepatol 5, 2.
- Warnes, T.W. and Smith, A. (1987) Baillieres Clin Gastroenterol 1, 63-89.
- Peng, S.Y. et al. (2004) Int J Cancer 112, 44-50.
- Meier, V. et al. (2006) Comp. Hepatol. 5, 2.
- Warnes, T.W. and Smith, A. (1987) Baillieres Clin. Gastroenterol. 1, 63-89.
- Peng, S.Y. et al. (2004) Int. J. Cancer 112, 44-50.
Application References
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Companion Products
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
