Cell Signaling Technology

Product Pathways - Development

AFP Antibody #2137

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M Recombinant 70 kDa Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

AFP Antibody detects recombinant human and mouse AFP protein. The antibody can only immunoprecipitate human AFP.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids near the carboxy terminus of human AFP. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of recombinant AFP, using AFP Antibody.

Background

Alpha-fetoprotein (AFP) is a 65 kDa glycoprotein found in the mammalian fetal liver, yolk sac, and GI tract. While AFP expression in adult cells is low, it is aberrantly expressed in adult liver cancer cells (1,2). The tumor suppressor gene p53 and β-catenin are both involved in the regulation of AFP expression. In normal adult cells, p53 binds to the repressor region of the AFP gene, thereby blocking transcription. Mutations in both p53 and β-catenin are associated with aberrant expression of AFP. Research studies have shown that elevated serum AFP levels are predictive of hepatocellular carcinoma (3).

  1. Meier, V. et al. (2006) Comp Hepatol 5, 2.
  2. Warnes, T.W. and Smith, A. (1987) Baillieres Clin Gastroenterol 1, 63-89.
  3. Peng, S.Y. et al. (2004) Int J Cancer 112, 44-50.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Products