Product Pathways - Glucose Metabolism
ATGL Antibody #2138
PhosphoSitePlus® protein, site, and accession data: PNPLA2
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP IHC-P IF-IC | M (R) | Endogenous | 54 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
M=Mouse
R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
ATGL Antibody detects endogenous levels of total ATGL protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to a sequence around Pro186 of human ATGL. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from NIH/3T3 and differentiated NIH/3T3-L1 cells, using ATGL antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded NIH/3T3-L1 cells undifferentiated (left) or differentiated (right), showing induced staining in adipocytes, using ATGL Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded mouse lung, showing specific staining of fat, using ATGL Antibody.
Background
Triglycerides form an important energy store in many living organisms. Adipose tissue serves as the primary storage depot for triglycerides in mammals. Lipolytic enzymes mobilize triglycerides during periods of starvation to provide organisms with necessary energy. Hormone-sensitive lipase (HSL), the first identified lipolytic enzyme, hydrolyzes triglycerides in mammalian adipose tissues (1-3). Additional lipolytic enzymes, including adipose triglyceride lipase (ATGL), have also been discovered. The primary function of ATGL is to catalyze the hydrolysis of the first ester bond of lipid molecules. This enzyme may provide diglyceride substrates for HSL hydrolysis. ATGL is abundantly expressed in murine white and brown adipose tissue, and is highly substrate specific (4). ATGL was independently identified as desnutrin (5) and the TG-hydrolace inducible phospholipase-A2-ζ (6).
- Holm, C. et al. (1988) Science 241, 1503-1506.
- Degerman, E. et al. (1990) Proc. Natl. Acad. Sci. USA 87, 533-537.
- Anthonsen, M.W. et al. (1998) J. Biol. Chem. 273, 215-221.
- Zimmermann, R. et al. (2004) Science 306, 1383-1386.
- Villena, J.A. et al. (2004) J. Biol. Chem. 279, 47066-47075.
- Jenkins, C.M. et al. (2004) J. Biol. Chem. 279, 48968-48975.
Application References
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- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.