Cell Signaling Technology

Product Pathways - Glucose Metabolism

ATGL Antibody #2138

Applications Reactivity Sensitivity MW (kDa) Source
W IP IHC-P IF-IC M (R) Endogenous 54 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

ATGL Antibody detects endogenous levels of total ATGL protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to a sequence around Pro186 of human ATGL. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 and differentiated NIH/3T3-L1 cells, using ATGL antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded NIH/3T3-L1 cells undifferentiated (left) or differentiated (right), showing induced staining in adipocytes, using ATGL Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse lung, showing specific staining of fat, using ATGL Antibody.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse brown fat, using ATGL Antibody in the presence of control peptide (left) or antigen-specific peptide (right).

IF-IC

IF-IC

Confocal immunofluorescent analysis of 3T3-L1 cells using ATGL Antibody (red) showing cytoplasmic localization in differentiated cells. Lipid droplets have been labeled with BODIPY 493/503 (green). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).

Background

Triglycerides form an important energy store in many living organisms. Adipose tissue serves as the primary storage depot for triglycerides in mammals. Lipolytic enzymes mobilize triglycerides during periods of starvation to provide organisms with necessary energy. Hormone-sensitive lipase (HSL), the first identified lipolytic enzyme, hydrolyzes triglycerides in mammalian adipose tissues (1-3). Additional lipolytic enzymes, including adipose triglyceride lipase (ATGL), have also been discovered. The primary function of ATGL is to catalyze the hydrolysis of the first ester bond of lipid molecules. This enzyme may provide diglyceride substrates for HSL hydrolysis. ATGL is abundantly expressed in murine white and brown adipose tissue, and is highly substrate specific (4). ATGL was independently identified as desnutrin (5) and the TG-hydrolace inducible phospholipase-A2-ζ (6).

  1. Holm, C. et al. (1988) Science 241, 1503-1506.
  2. Degerman, E. et al. (1990) Proc. Natl. Acad. Sci. USA 87, 533-537.
  3. Anthonsen, M.W. et al. (1998) J. Biol. Chem. 273, 215-221.
  4. Zimmermann, R. et al. (2004) Science 306, 1383-1386.
  5. Villena, J.A. et al. (2004) J. Biol. Chem. 279, 47066-47075.
  6. Jenkins, C.M. et al. (2004) J. Biol. Chem. 279, 48968-48975.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

Products