Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

LSD1 Antibody #2139

Applications Reactivity Sensitivity MW (kDa) Source
W IP IHC-P IF-IC F H M R Mk Endogenous 110 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

LSD1 Antibody detects endogenous levels of total LSD1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino-terminus of human LSD1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of cell lysates from various cell lines, using LSD1 Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma showing nuclear localization, using LSD1 Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical staining of paraffin-embedded human breast carcinoma, using LSD1 Antibody preincubated with control peptide (left) or antigen-specific peptide (right).


Flow Cytometry

Flow Cytometry

Flow cytometric analysis of untreated HeLa cells, using LSD1 Antibody (blue) compared to a nonspecific negative control antibody (red).

IF-IC

IF-IC

Immunofluorescent analysis of HeLa cells, using LSD1 Antibody (right). Nuclei are stained with DAPI (left).

Background

Lysine-specific demethylase 1 (LSD1; also known as AOF2 and BHC110) is a nuclear amine oxidase homolog that acts as a histone demethylase and transcription cofactor (1). Gene activation and repression is specifically regulated by the methylation state of distinct histone protein lysine residues. For example, methylation of histone H3 at Lys4 facilitates transcriptional activation by coordinating the recruitment of BPTF, a component of the NURF chromatin remodeling complex, and WDR5, a component of multiple histone methyltransferase complexes (2,3). In contrast, methylation of histone H3 at Lys9 facilitates transcriptional repression by recruiting HP1 (4,5). LSD1 is a component of the CoREST transcriptional co-repressor complex that also contains CoREST, CtBP, HDAC1 and HDAC2. As part of this complex, LSD1 demethylates mono-methyl and di-methyl histone H3 at Lys4 through a FAD-dependent oxidation reaction to facilitate neuronal-specific gene repression in non-neuronal cells (1,6,7). In contrast, LSD1 associates with androgen receptor in human prostate cells to demethylate mono-methyl and di-methyl histone H3 at Lys9 and facilitate androgen receptor-dependent transcriptional activation (8). Therefore, depending on gene context LSD1 can function as either a transcriptional co-repressor or co-activator. LSD1 activity is inhibited by the amine oxidase inhibitors pargyline, deprenyl, clorgyline and tranylcypromine (8).

  1. Shi, Y. et al. (2004) Cell 119, 941-953.
  2. Wysocka, J. et al. (2006) Nature 442, 86-90.
  3. Wysocka, J. et al. (2005) Cell 121, 859-872.
  4. Jacobs, S.A. and Khorasanizadeh, S. (2002) Science 295, 2080-2083.
  5. Nielsen, P.R. et al. (2002) Nature 416, 103-107.
  6. Shi, Y.J. et al. (2005) Mol. Cell 19, 857-864.
  7. Lee, M.G. et al. (2005) Nature 437, 432-435.
  8. Metzger, E. et al. (2005) Nature 437, 436-439.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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