Cell Signaling Technology

Product Pathways - Translational Control

Rictor Antibody #2140

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Mk Endogenous 200 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Rictor Antibody detects endogenous levels of total rictor protein.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) derived from the sequence around Lys1125 of human rictor. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines, using Rictor Antibody.

Background

Cell growth is a fundamental biological process whereby cells accumulate mass and increase in size. The mammalian TOR (mTOR) pathway regulates growth by coordinating energy and nutrient signals with growth factor-derived signals (1). mTOR is a large protein kinase with two different complexes. One complex contains mTOR, GβL and raptor, which is a target of rapamycin. The other complex, insensitive to rapamycin, includes mTOR, GβL and rictor (1). The mTOR-rictor complex phosphorylates Ser473 of Akt/PKB in vitro (2). This phosphorylation is essential for full Akt/PKB activation. Furthermore, an siRNA knockdown of rictor inhibits Ser473 phosphorylation in 3T3-L1 adipocytes (3). This complex has also been shown to phosphorylate the rapamycin-resistant mutants of S6K1, another effector of mTOR (4).

  1. Sarbassov, D.D. et al. (2004) Curr. Biol. 14, 1296-1302.
  2. Sarbassov, D.D. et al. (2005) Science 307, 1098-1101.
  3. Hresko, R.C. and Mueckler, M. (2005) J. Biol. Chem. 280, 40406-40416.
  4. Ali, S.M. and Sabatini, D.M. (2005) J. Biol. Chem. 280, 19445-19448.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

Products