Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Rab5 Antibody #2143

Applications Reactivity Sensitivity MW (kDa) Source
W IF-IC H M R Hm Mk Endogenous 25 Rabbit

Applications Key:  W=Western Blotting  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Hm=Hamster  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Rab5 Antibody detects endogenous levels of total Rab5A protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly190 of human Rab5A protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from MCF-7, Neuro2A, PC12 and COS cells, using Rab5 Antibody.

IF-IC

IF-IC

Confocal immunofluorescent analysis of MCF-7 cells using Rab5 Antibody (green). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).

Background

Rab5 is a member of the Ras superfamily of small Rab GTPases. Rab5 is localized at the plasma membrane and early endosomes and functions as a key regulator of vesicular trafficking during early endocytosis (1). The conformational change between Rab5 GTP/GDP states is essential for its biological function as a rate limiting regulator at multiple steps during endocytosis (1,2). Rab5 exerts its function by interacting with several Rab5-specific effectors (1-3). These proteins form complexes with Rab5 on a specialized Rab domain of the endosome and promote recycling of Rab5-cargo targets between endosome and the plasma membrane.

  1. Zerial, M. and McBride, H. (2001) Nat Rev Mol Cell Biol 2, 107-17.
  2. van der Bliek, A.M. (2005) Nat Cell Biol 7, 548-50.
  3. Haas, A.K. et al. (2005) Nat Cell Biol 7, 887-93.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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