Product Pathways - Cytoskeletal Signaling
Rab5 Antibody #2143
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IF-IC | H M R Mk Hm | Endogenous | 25 | Rabbit |
Applications Key:
W=Western Blotting
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Hm=Hamster
Species cross-reactivity is determined by Western blot.
Protocols
Specificity / Sensitivity
Rab5 Antibody detects endogenous levels of total Rab5 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding residue surrounding Gly190 of human Rab5 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from MCF-7, Neuro2A, PC12 and COS cells, using Rab5 Antibody.
IF-IC
Confocal immunofluorescent analysis of MCF-7 cells using Rab5 Antibody (green). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
Background
Rab5 is a member of the Ras superfamily of small Rab GTPases. Rab5 is localized at the plasma membrane and early endosomes and functions as a key regulator of vesicular trafficking during early endocytosis (1). The conformational change between Rab5 GTP/GDP states is essential for its biological function as a rate limiting regulator at multiple steps during endocytosis (1,2). Rab5 exerts its function by interacting with several Rab5-specific effectors (1-3). These proteins form complexes with Rab5 on a specialized Rab domain of the endosome and promote recycling of Rab5-cargo targets between endosome and the plasma membrane.
- Zerial, M. and McBride, H. (2001) Nat. Rev. Mol. Cell Biol. 2, 107-117.
- van der Bliek, A.M. (2005) Nat. Cell Biol. 7, 548-550.
- Haas, A.K. et al. (2005) Nat. Cell Biol. 7, 887-893.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
