Product Pathways - Cytoskeletal Signaling
NSF Antibody #2145
|2145S||100 µl (10 western blots)||---||In Stock||---|
|2145||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat, Hamster, Monkey||Endogenous||78||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Specificity / Sensitivity
NSF Antibody detects endogenous levels of total NSF protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu524 of human NSF. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from SHSY5Y, Neuro2A, PC12 and CHO cells using NSF Antibody.
Several protein-protein interactions are essential to membrane fusion during endocytosis. Membrane fusion requires interaction among SNARE1 proteins associated with both donor and acceptor membranes (1,2). Following membrane fusion, the α-SNAP cytoplasmic adapter protein binds to the SNARE complex. N-ethylmaleimide-sensitive factor (NSF), a hexameric ATPase, then associates with the α-SNAP/SNARE complex to mediate SNARE disassembly during membrane fusion (3,4). The ATPase activity of NSF induces a conformational change in the α-SNAP/SNARE complex that leads to its dissociation from the membrane, membrane fusion and eventual recycling of the SNARE complex for subsequent membrane fusion (3,4).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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