Product Pathways - Cytoskeletal Signaling
NSF Antibody #2145
PhosphoSitePlus® protein, site, and accession data: NSF
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP IF-F | H M R Hm Mk | Endogenous | 78 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IF-F=Immunofluorescence (Frozen)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Hm=Hamster
Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
NSF Antibody detects endogenous levels of total NSF protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu524 of human NSF. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from SHSY5Y, Neuro2A, PC12 and CHO cells using NSF Antibody.
IP
Immunoprecipitation of NSF from untreated HeLa cell extracts using NSF antibody (Lane 3). Lane 1: Input control. Lane 2: No antibody control.
IF-F
Confocal immunofluorescent analysis of normal rat brain using NSF Antibody (red) and GFAP (GA5) Mouse mAb #3670 (green) showing cytoplasmic localization in neurons in the deep cerebellar nuclei (left) and the pons (right). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).
Background
Several protein-protein interactions are essential to membrane fusion during endocytosis. Membrane fusion requires interaction among SNARE1 proteins associated with both donor and acceptor membranes (1,2). Following membrane fusion, the α-SNAP cytoplasmic adapter protein binds to the SNARE complex. N-ethylmaleimide-sensitive factor (NSF), a hexameric ATPase, then associates with the α-SNAP/SNARE complex to mediate SNARE disassembly during membrane fusion (3,4). The ATPase activity of NSF induces a conformational change in the α-SNAP/SNARE complex that leads to its dissociation from the membrane, membrane fusion and eventual recycling of the SNARE complex for subsequent membrane fusion (3,4).
- Ungermann, C. and Langosch, D. (2005) J Cell Sci 118, 3819-28.
- Leabu, M. (2006) J Cell Mol Med 10, 423-7.
- May, A.P. et al. (2001) J Biol Chem 276, 21991-4.
- Dalal, S. et al. (2004) Mol Biol Cell 15, 637-48.
Application References
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
