Product Pathways - Cytoskeletal Signaling
α/β-Tubulin Antibody #2148
| Applications | Reactivity | MW (kDa) | Source |
|---|---|---|---|
| W IHC-P IF-IC F | H M R Mk B | 55 | Rabbit |
Applications Key:
W=Western Blotting
IHC-P=Immunohistochemistry (Paraffin)
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
B=Bovine
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
The α/β-Tubulin Antibody detects endogenous levels of both α- and β-tubulin total protein, and does cross-react with both recombinant α- and β-tubulin.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to the sequence of human α- and β-tubulin. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from HeLa, NIH/3T3, C6 and COS-7 cells, using α/β-Tubulin Antibody.
Western Blotting
Western blot analysis of recombinant alpha-tubulin and beta-tubulin GST-fusion proteins, and extracts from NIH/3T3 and C6 cells, using α/β-Tubulin Antibody (left) and GST Antibody #2622 (right).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using α/β-Tubulin Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, showing cytoplasmic localization, using α/β-Tubulin Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human MALToma using α/β-Tubulin Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using β-Tubulin (9F3) Rabbit mAb #2128 in the presence of control peptide (left) or β-Tubulin Blocking Peptide (right).
Flow Cytometry
Flow cytometric analysis of C6 cells using α/β-Tubulin Antibody (blue) compared to a nonspecific negative control antibody (red).
IF-IC
Confocal immunofluorescent analysis of NIH-3T3 cells, using α/β-Tubulin Antibody (green) and Phospho-Histone H3 (Ser10) (6G3) Mouse mAb #9706 (red) showing different stages of the cell cycle.
IF-IC
Confocal microscopic images of NIH/3T3 cells showing cytoskeletal stain with α/β-Tubulin Antibody (A) compared to an isotype control (B).
Background
The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is necessary to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1).
Application References
- Marini, F. et al. (2006) Proc Natl Acad Sci U S A 103, 17325-30. This article references the use of α/β-Tubulin Antibody in the following applications: Western Blotting
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Companion Products
- 2144 α-Tubulin Antibody
- 2146 β-Tubulin Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 2125 α-Tubulin (11H10) Rabbit mAb
- 2128 β-Tubulin (9F3) Rabbit mAb
