Cell Signaling Technology

Product Pathways - Neuroscience

TorsinA (D-M2A8) Mouse mAb #2150

Applications Reactivity Sensitivity MW (kDa) Isotype
W H M R Endogenous 35 Mouse IgG1

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Specificity / Sensitivity

TorsinA (D-M2A8) Mouse mAb detects endogenous levels of total torsinA protein. TorsinA (D-M2A8) Mouse mAb does not detect TorsinB (Hewett, J. et al., 2004).

Source / Purification

Monoclonal antibody is produced by immunizing animals with human recombinant torsinA (aa 51-324).

Western Blotting

Western Blotting

Western blot analysis of extracts from SH-SY5Y and U87MG cells, using TorsinA (D-M2A8) Mouse mAb.

Background

The neurological condition Dystonia is associated with sustained muscle contractions and abnormal posturing (1). TorsinA, torsinB, torp2A and torp3A belong to the family of ATPases associated with cellular activites (AAA+) and mutations in torsinA cause early onset dystonia (2). TorsinA has been shown to suppress intracellular protein aggregation in C. elegans and possesses chaperon activity. Interestingly, torsinA is highly expressed in dopaminergic neurons and associates with alpha-synuclein in Lewy bodies, which pathologically characterize Parkinson's Disease (3-5).

  1. Fahn, S. (1988) Adv. Neurol. 50, 1-8.
  2. Ozelius, L.J. et al. (1997) Nat. Genet. 17, 40-8.
  3. Caldwell, G.A. et al. (2003) Hum. Mol. Genet. 12, 307-19.
  4. McLean, P.J. et al. (2002) J. Neurochem. 83, 846-54.
  5. O'Farrell, C. et al. (2004) Brain Res. Mol. Brain Res. 127, 1-9.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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