Product Pathways - Wnt / Hedgehog / Notch
Axin2 (76G6) Rabbit mAb #2151
| Applications | Reactivity | MW (kDa) | Source | Isotype |
|---|---|---|---|---|
| W IP | H | 95, 98 | Rabbit | IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Axin2 (76G6) Rabbit mAb detects endogenous levels of total Axin2 protein. It does not cross-react with Axin1.
Source / Purification
Monoclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues surrounding Pro566 of human Axin2.
Western Blotting
Western blot analysis of total cell lysates from HCT15 and SW620 cells, using Axin2 (76G2) Rabbit mAb.
Background
Axin1 (Axis inhibition protein 1) and Axin2 are multidomain scaffold proteins that negatively regulate Wnt signaling. Axin1 complexes with APC, GSK-3β, Dvl and β-catenin and promotes the GSK-3β-mediated phosphorylation and subsequent degradation of β-catenin (1,2). Upon stimulation of cells with Wnt, Axin1 is recruited to the membrane by phosphorylated LRP5/6, a process that is believed to be crucial for activation of Wnt signaling (3,4). In addition to its role in the Wnt signaling pathway, Axin1 complexes with MEKK1 and causes the activation of c-Jun amino-terminal kinase (JNK/SAPK) (5). Axin2 (also known as Conductin or Axil) can functionally substitute for Axin1 in mice (6). Axin2 itself is a direct target of the Wnt signaling pathway and therefore serves to control the duration and/or intensity of Wnt signaling through a negative feedback loop (7-9).
- Luo, W. and Lin, S.C. Neurosignals 13, 99-113.
- Salahshor, S. and Woodgett, J.R. (2005) J. Clin. Pathol. 58, 225-236.
- Zeng, X. et al. (2005) Nature 438, 873-877.
- Davidson, G. et al. (2005) Nature 438, 867-872.
- Zhang, Y. et al. (1999) J. Biol. Chem. 274, 35247-35254.
- Chia, I.V. and Costantini, F. (2005) Mol. Cell Biol. 25, 4371-4376.
- Jho, E.H. et al. (2002) Mol. Cell Biol. 22, 1172-1183.
- Lustig, B. et al. (2002) Mol. Cell Biol. 22, 1184-1193.
- Leung, J.Y. et al. (2002) J. Biol. Chem. 277, 21657-21665.
Application References
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Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
