Product Pathways - Cytoskeletal Signaling
Vav1 (R775) Antibody #2153
PhosphoSitePlus® protein, site, and accession data: VAV1
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | H | Endogenous | 95 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
Vav1 (R775) Antibody detects endogenous levels of total Vav1 protein. The antibody does not cross-react with Vav2 or Vav3.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding the carboxy terminus of human Vav1. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from various cell types, using Vav1 (R775) Antibody.
IP
Immunoprecipitation of Vav1 from Jurkat cell lysates, using Vav1 (R775) Antibody. Western blot was performed using the same antibody.
Background
Vav proteins belong to the Dbl family of guanine nucleotide exchange factors (GEFs) for Rho/Rac small GTPases. The three identified mammalian Vav proteins (Vav1, Vav2 and Vav3) differ in their expression. Vav1 is expressed only in hematopoietic cells and is involved in the formation of the immune synapse. Vav2 and Vav3 are more ubiquitously expressed. Vav proteins contain the Dbl homology domain, which confers GEF activity, as well as protein interaction domains that allow them to function in pathways regulating actin cytoskeleton organization (reviewed in 1). Phosphorylation stimulates the GEF activity of Vav protein towards Rho/Rac (2,3).
- Hornstein, I. et al. (2004) Cell. Signal. 16, 1-11.
- Crespo, P. et al. (1997) Nature 385, 169-172.
- Han, J. et al. (1997) Mol. Cell. Biol. 17, 1346-1353.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
