Cell Signaling Technology

Product Pathways - Neuroscience

Musashi Antibody #2154

Applications Reactivity MW (kDa) Source
W IF-F H M R 35 Rabbit

Applications Key:  W=Western Blotting  IF-F=Immunofluorescence (Frozen)
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Musashi Antibody detects endogenous levels of total Musashi 1 and 2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues of human Musashi. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from IEC-6 and LS174T cells, using Musashi Antibody.

IF-F

IF-F

Confocal immunofluorescence images of the subventricular zone of rat striatal sections labeled with Musashi Antibody (red) and Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb (Alexa Fluor® 488 Conjugate) #4854 (green). Proliferating/mitotic cells are labeled with Phospho-Histone H3 (Ser10) (6G3) Mouse mAb #9706 (blue).

Background

Musashi-1 and Musashi-2 are RNA-binding proteins which play a role in asymmetric cell division of ectodermal precursor cells by regulating the translation of target mRNA. Both family members augment Notch signaling and repress the translation of m-Numb, a protein that positively modulates differentiation of neural stem cells into neurons. Thus, Musashi contributes to the maintenance of neural stem cells (1). While Musashi-1 is frequently used as a marker for proliferating neural precursor cells, it is also expressed in epithelial stem cells including intestinal and mammary gland stem cells (2-4).

  1. Okano, H. et al. (2005) Exp. Cell Res. 306, 349-56.
  2. Sakakibara, S. et al. (1996) Dev. Biol. 176, 230-42.
  3. Potten, C.S. et al. (2003) Differentiation 71, 28-41.
  4. Clarke, R.B. et al. (2005) Dev. Biol. 277, 443-56.

Application References

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