Product Pathways - Adhesion
α-N-Catenin (C12G4) Rabbit mAb #2163
| Applications | Reactivity | Sensitivity | MW (kDa) | Source | Isotype |
|---|---|---|---|---|---|
| W IP IF-IC | H M R | Endogenous | 102 | Rabbit | IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
α-N-Catenin (C12G4) Rabbit mAb detects endogenous levels of total α-N-catenin protein. The antibody does not cross-react with other α-catenin family members.
Source / Purification
Monoclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to the carboxy-terminal sequence of human α-N-Catenin.
Western Blotting
Western blot analysis of extracts from SH-SY5Y cells, mouse brain and rat brain using α-N-Catenin (C12G4) Rabbit mAb.
IP
Immunoprecipitation of α-N-Catenin from SH-SY5Y cell lysates using α-N-Catenin (C12G4) Rabbit mAb. Western blot was performed using the same antibody.
IF-IC
Confocal immunofluorescent analysis of SH-SY5Y cells using α-N-Catenin (C12G4) Rabbit mAb (green) and S6 Ribosomal Protein (54D2) Mouse mAb #2317 (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
Background
Adherens junctions are dynamic structures that form cell-cell contacts and are important in development, differentiation, tissue integrity, morphology and cell polarity. They are composed of the transmembrane proteins, cadherins, which bind cadherins on adjacent cells in a calcium-dependent manner. On the cytoplasmic side of adherens junctions, the classic model states that cadherins are linked to the cytoskeleton through β- and α-catenin. α-E-catenin is ubiquitously expressed, α-N-catenin is expressed in neuronal tissue, and α-T-catenin is primarily expressed in heart tissue. Loss of E-cadherin and α-E-catenin occurs during the progression of several human cancers, indicating that the breakdown of adherens junctions is important in cancer progression (reviewed in 1).Recent evidence suggests that, rather than acting as a static link between cadherins and actin, α-catenin regulates actin dynamics directly, possibly by competing with the actin nucleating arp2/3 complex (2,3). α-catenin also plays a role in regulating β-catenin-dependent transcriptional activity, affecting differentiation and response to Wnt signaling. α-catenin binds to β-catenin in the nucleus, preventing it from regulating transcription, and levels of both proteins appear to be regulated via proteasome-dependent degradation (4).
- Kobielak, A. and Fuchs, E. (2004) Nat. Rev. Mol. Cell Biol. 5, 614-625.
- Yamada, S. et al. (2005) Cell 123, 889-901.
- Drees, F. et al. (2005) Cell 123, 903-915.
- Hwang, S.G. et al. (2005) J. Biol. Chem. 280, 12758-12765.
Application References
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
