Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

EB-1 (1A11/4) Mouse mAb #2164

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H Mk Endogenous 30 Mouse IgG2b

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

EB-1 (1A11/4) Mouse mAb detects endogenous levels of EB-1.

Source / Purification

Monoclonal antibody is produced by immunizing CD-1 mice with a recombinant human EB-1 protein.

Western Blotting

Western Blotting

Western blot analysis of total cell lysates from HeLa and COS cells, using EB-1 Antibody.

Background

EB1 (end-binding protein 1) is a microtubule associated protein (1). EB1 localizes to the growing ends of microtubules, the centrosome and the mitotic spindle (2-4). EB1 is also found to associate with the adenomatous polyposis coli (APC) protein (5). Recent studies also suggest that EB1 plays a role in microtubule-based transport (6).

  1. Tirnauer, J.S. and Bierer, B.E. (2000) J. Cell Biol. 149, 761-766.
  2. Vaughan, K.T. (2005) J. Cell Biol. 171, 197-200.
  3. Berrueta, L. et al. (1998) Proc. Natl. Acad. Sci. USA 95, 10596-10601.
  4. Morrison, E.E. et al. (1998) Oncogene 17, 3471-3477.
  5. Su, L.K. et al. (1995) Cancer Res. 55, 2972-2977.
  6. Vaughan, K.T. (2005) J. Cell Biol. 171, 197-200.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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