Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Rab4 Antibody #2167

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Hm Mk Endogenous 25 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Hm=Hamster  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Rab4 Antibody detects endogenous levels of total Rab4 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp128 of human Rab4 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using Rab4 Antibody.

Background

Rab4 is a member of the Ras superfamily of small Rab GTPases implicated in endocytosis. Rab4 is localized at early endosomes/recycling endosomes and functions as a key regulator for sorting/recycling of membrane and proteins (1,2). Rab4 has two isoforms, Rab4A and Rab4B, both of which are localized in similar cellular compartments and are believed to have similar functions (4). Rab4 interacts with several Rab4 effectors in a complex on a special endosome site that promotes membrane/protein recycling (1,3).

  1. Zerial, M. and McBride, H. (2001) Nat. Rev. Mol. Cell Biol. 2, 107-117.
  2. van der Sluijs, P. et al. (1992) Cell 70, 729-740.
  3. Deneka, M. et al. (2003) EMBO J. 22, 2645-2657.
  4. Krawczyk, M. et al. (2007) Nucleic Acids Res. 35, 595-605.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

Products