Product Pathways - Growth Factors/Cytokines
Interleukin-11 #2168
| MW | Source | Purity |
|---|---|---|
| 19.1 kDa | Human recombinant protein |
Description
The human IL-11 coding cDNA was subcloned into an expression vector and expressed in E. coli. The recombinant human IL-11 proteins were purified and stored in PBS buffer containing 0.1% BSA.
Concentration and Specific Activity
100 μg/ml, 5 x 10 (6) IU/mg
Western Blotting
Western blot analysis of extracts from HT29 cells, untreated or IL-11-stimulated (100 ng/ml for 15 min), using Phospho-Stat3 (Tyr705) (D3A7) Rabbit mAb #9145 (upper) or Stat3 (79D7) Rabbit mAb #4904 (lower).
Directions for Use
CST recommends using 50-100 ng/ml of IL-11 for stimulation of IL-11 signaling.
Background
TBA
Interleukin-11 (IL-11) was initially cloned as a mediator of plasmacytoma cell proliferation and was later found to exhibit a wide variety of biological effects in neural cells as well as in the hematopoietic and immune systems (1). IL-11 belongs to the interleukin-6 (IL-6)-type subfamily of long-chain helical cytokines including IL-6, ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), oncostatin M and cardiotrophin-1, which all share the glycoprotein gp130 as a signal transducing receptor component. IL-11 acts on cells expressing gp130 and the IL-11 receptor (IL-11R) α subunit. Both receptor subunits belong to the family of class I cytokine receptors. The complex of IL-11 and IL-11R triggers the activation of gp130 most likely by enforcing gp130 homodimerization (2). As a consequence of gp130 activation several cytoplasmic signal transduction cascades are initiated from which the Janus kinase (Jak)/signal transducer and activator of transcription (Stat) pathway has attracted considerable attention. Initiation of the Jak/Stat pathway in response to IL-11 requires Jak1 and leads predominantly to the activation of Stat3 (3,4).
- Du, X. and Williams, D.A. (1997) Blood 89, 3897-3908.
- Tacken, I. et al. (1999) Eur. J. Biochem. 265, 645-655.
- Dahmen, H. et al. (1998) Biochem J. 331 ( Pt 3), 695-702.
- Kiessling, S. et al. (2004) J. Biol. Chem. 279, 10304-10315.
Application References
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