Cell Signaling Technology

Product Pathways - Apoptosis

Alix (3A9) Mouse mAb #2171

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M R Mk Endogenous 95 Mouse IgG1

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Alix (3A9) Mouse mAb detects endogenous levels of total Alix protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with full-length recombinant human Alix protein.

Western Blotting

Western Blotting

Western blot analysis of cell extracts from various cell types using Alix (3A9) Mouse mAb.

Background

Alix, a phylogenetically conserved cytosolic scaffold protein, contains an N-terminal Bro1 domain, a coiled-coil region and a C-terminal proline-rich domain (1,2). Originally identified as an ALG-2 (apoptosis-linked gene 2)-interacting protein involved in programmed cell death (3,4), Alix also regulates many other cellular processes, such as endocytic membrane trafficking and cell adhesion through interactions with ESCRT (endosomal sorting complex required for transport) proteins, endophilins, and CIN85 (Cbl-interacting protein of 85 kDa) (5,6).

  1. Mattei, S. et al. (2006) Eur. J. Cell Biol. 85, 925-936.
  2. Odorizzi, G. (2006) J. Cell Sci. 119, 3025-3032.
  3. Vito, P. et al. (1999) J. Biol. Chem. 274, 1533-1540.
  4. Missotten, M. et al. (1999) Cell Death Differ. 6, 124-129.
  5. Katoh, K. et al. (2003) J. Biol. Chem. 278, 39104-39113.
  6. Sadoul, R. (2006) Biol. Cell 98, 69-77.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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