Product Pathways - Neuroscience
Calbindin (C26D12) Rabbit mAb #2173
PhosphoSitePlus® protein, site, and accession data: Calb1
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IHC-P | H M R | Endogenous | 28 | Rabbit IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
Calbindin (C26D12) Rabbit mAb detects endogenous levels of total calbindin protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding amino acid 200 of human calbindin.
Western Blotting
Western blot analysis of extracts from mouse and rat brain using Calbindin (C26D12) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded mouse cerebellum using Calbindin (C26D12) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
Background
Calcium-binding proteins of different subfamilies regulate the second messenger calcium. Calbindin, calmodulin, S-100, paravalbumin and troponin C are members of the low molecular weight calcium-binding protein family (1). Calbindin is expressed in discrete neuronal populations within the CNS and is thought to act as an intracellular calcium buffering protein. Most Purkinje cells express calbindin, which is expressed when neurons start to migrate and differentiate. In contrast, other calcium buffering proteins, such as parvalbumin, are expressed later during development and in parallel with increasing neuronal activity (2).
Application References
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For Research Use Only. Not For Use In Diagnostic Procedures.
