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Cyclophilin A Antibody #2175

Applications	Reactivity	Sensitivity	MW (kDa)	Source
W	H M R Mk (B) (Dg)	Endogenous	18	Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  B=Bovine  Dg=Dog
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

2175:

Western Blotting

Specificity / Sensitivity

Cyclophilin A Antibody detects endogenous levels of total Cyclophilin A protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of Cyclophilin A. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Cyclophilins are a highly conserved family of peptidylprolyl cis-trans-isomerases (PPIA) that are targets of the immunosuppressant drug cyclosporin A (CsA) (1,2). The complex of cyclophilin and CsA can bind to and inhibit calcineurin which leads to inhibition of the transcription factor NFAT and decreased production of cytokines (3,4). As isomerases, cyclophilins have been proposed to aid in protein folding. Cyclophilin A can bind to the p55 Gag protein of HIV and appears necessary for HIV infection (5,6). There is also some evidence that cyclophilins have nuclease activity and play a role in apoptosis (7).

1. Takahashi, N. et al. (1989) Nature 337, 473-475.
2. Wang, P. and Heitman, J. (2005) Genome Biol. 6, 226.
3. Jain, J. et al. (1993) Nature 365, 352-355.
4. McCaffrey, P.G. et al. (1993) J. Biol. Chem. 268, 3747-3752.
5. Thali, M. et al. (1994) Nature 372, 363-365.
6. Streblow, D.N. et al. (1998) Virology 245, 197-202.
7. Montague, J.W. et al. (1994) J. Biol. Chem. 269, 18877-18880.

Application References

• Dear, J.W. et al. (2011) J Immunol 187, 3347-52. Applications: Western Blotting

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Companion Products

• 2614 Pan-Calcineurin A Antibody
• 4998 NFAT4 Antibody
• 7071 Phototope^®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide

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For Research Use Only. Not For Use In Diagnostic Procedures.