Cell Signaling Technology

Product Pathways - Metabolism

PGC-1α (3G6) Rabbit mAb #2178

Applications Reactivity Sensitivity MW (kDa) Isotype
W H Transfected Only 130 Rabbit IgG

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

PGC-1α (3G6) Rabbit mAb detects transfected levels of total PGC-1α protein. This antibody does not cross-react with PGC-1β or other related proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant human PGC-1α fusion protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, either mock transfected (-) or transfected with a construct expressing full-length Myc-DDK-tagged human wild-type PGC-1α (+), using PGC-1α (3G6) Rabbit mAb.

Background

PPARγ coactivator-1α (PGC-1α) was originally identified as a transcriptional coactivator whose expression closely correlated with adaptive thermogenesis following exposure to cold temperatures (1). Named for its association with the nuclear receptor peroxisome-proliferator activated receptor (PPARγ), PGC-1α interacts with a diverse array of transcription factors to regulate numerous aspects of cell physiology (2). PGC-1α helps to regulate cell processes important in adaptive thermogenesis and energy metabolism, including the related functions of glucose uptake, gluconeogenesis, insulin secretion, and mitochondrial biogenesis (3). Long thought to be a potential therapeutic target for the treatment of type II diabetes, obesity, cardiomyopathy, or other metabolic disorders (reviewed in 4), a recent functional survey found no obvious differences in PPARγ activity associated with recognized PGC-1α variants (5).

  1. Puigserver, P. et al. (1998) Cell 92, 829-839.
  2. Liang, H. and Ward, W.F. (2006) Adv. Physiol. Educ. 30, 145-151.
  3. Tiraby, C. and Langin, D. (2005) Med. Sci. (Paris) 21, 49-54.
  4. Finck, B.N. and Kelly, D.P. (2006) J. Clin. Invest. 116, 615-622.
  5. Nitz, I. et al. (2007) Biochem. Biophys. Res. Commun. 353, 481-486.

Application References

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Companion Products

Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.

For Research Use Only. Not For Use In Diagnostic Procedures.

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