Cell Signaling Technology

Product Pathways - Apoptosis

eIF4G2/p97 Antibody #2182

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Endogenous 97 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

eIF4G2/p97 Antibody detects endogenous levels of total eIF4G2/p97 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu805 of human eIF4G2/p97 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from human (Jurkat and HeLa), mouse (NIH/3T3 and L929) and rat (C6 and PC12) cells, using eIF4G2/p97 Antibody.

Background

The initiation of translation is an important biological event and a variety of factors contribute to this process. Members of the eIF4 translation initiation factor family bind to the 5' m7GTP mRNA cap and unwind the mRNA secondary structure (1,2). The amino-terminal portion of eIF4G physically associates with eIF4E to stimulate the binding of eIF4E to the mRNA cap structure (3). eIF4G also interacts with eIF3 and eIF4A and serves as an adaptor molecule in the eIF4 complex (4). Moreover, eIF4G plays a role in internal ribosomal entry site (IRES)-mediated initiation of translation (5,6). The eIF4G family includes eIF4G1 (eIF4GI), eIF4G2 (p97, DAP5 or NAT1), and eIF4G3 (eIF4GII) (7). These factors share a homologous sequence that provides for interaction with initiation factors eIF3 and eIF4A. Both eIF4G1 and eIF4G3 are involved in cap-dependent translation, while eIF4G2 plays a role in IRES-mediated translation of some genes during cell stress (7,8).

  1. Yan, R. and Rhoads, R.E. (1995) Genomics 26, 394-398.
  2. Morley, S.J. et al. (1997) RNA 3, 1085-1104.
  3. Haghighat, A. and Sonenberg, N. (1997) J. Biol. Chem. 272, 21677-21680.
  4. De Gregorio, E. et al. (1998) RNA 4, 828-836.
  5. Ohlmann, T. et al. (1996) EMBO J. 15, 1371-1382.
  6. Borman, A.M. and Kean, K.M. (1997) Virology 237, 129-136.
  7. Henis-Korenblit, S. et al. (2002) Proc. Natl. Acad. Sci. USA 99, 5400-5405.
  8. Nevins, T.A. et al. (2003) J. Biol. Chem. 278, 3572-3579.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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