Cell Signaling Technology

Product Pathways - Apoptosis / Autophagy

DAP5 Antibody #2182

Applications Reactivity MW (kDa) Source
W H M R 97 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

DAP5 Antibody detects endogenous levels of total DAP5 protein. The antibody does not cross-react with other DAP family members or with eIF4G.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH coupled) corresponding to residues surrounding amino acid 805 of human DAP5. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from human (Jurkat and HeLa), mouse (NIH/3T3 and L929) and rat (C6 and PC12) cells, using DAP5 Antibody.

Background

Death associated protein 1 (DAP1) is a 15 kDa protein that functions as a positive mediator of cell death initiated by interferon-gamma (1,2). The DAP1 protein is proline rich and possesses one SH3 binding motif, as well as several consensus protein kinase phosphorylation sites (1). The protein is localized in the cytoplasm, but the detailed mechanism of its proapoptotic function is unclear. Death associated protein 3 (DAP3) is widely expressed, and the expression is upregulated during membrane receptor-mediated apoptosis. In interferon-gamma- and Fas-induced apoptosis, DAP3 acts as a positive mediator, functioning downstream of the receptor signaling complex and upstream of the effector caspases (3,4). Death associated protein 5 (DAP5) is a 97 kDa protein with a high degree of amino acid sequence homology to eukaryotic translation initiation factor 4G (Elf4G) (1,5). Compared with elF4G, DAP5 lacks the amino-terminal region necessary for cap-dependent translation, and has a unique carboxy-terminal region that functions as a regulator of interferon-gamma-induced cell death (5,6). During induction of apoptosis, DAP5 is cleaved at aspartic acid 790. The carboxy-terminal truncated form of DAP5 functions as a cap-independent translation initiation factor responsible for the mediation of its own translation during apoptosis (7).

  1. Deiss, L. P. et al. (1995) Genes Dev. 9, 15-30.
  2. Levy-Strumpf, N. and Kimchi, A. (1998) Oncogene 17, 3331-3340.
  3. Kissil, J. L. et al. (1995) J. Biol. Chem. 270, 27932-27936.
  4. Kissil, J. L. et al. (1999) EMBO J. 18, 353-362.
  5. Imataka, H. et al. (1997) EMBO J. 16, 817-825.
  6. Levy-Strumpf, N. et al. (1997) Mol. Cell. Biol. 17, 1615-1625.
  7. Henis-Korenblit, S. et al. (2000) Mol. Cell. Biol. 20, 496-506.

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