Cell Signaling Technology

Product Pathways - Translational Control

YY1 (13G10) Rabbit mAb #2185

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M R Endogenous 65 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

YY1 (13G10) Rabbit mAb detects endogenous levels of total YY1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding amino acid 175 of human YY1.

Western Blotting

Western Blotting

Western blot analysis of total cell lysates from C6 and PC12 cells, using YY1 (13G10) Rabbit mAb.

Background

YY1 (Yin Yang1) is a ubiquitously expressed transcription factor with fundamental roles in embryogenesis, differentiation, replication and proliferation. YY1 contains four zinc finger motifs of the Cys-Cys-His-His type and can activate different eukaryotic genes (such as CREB, c-myc, Histone H4, p53 and PARP-1) or repress different eukaryotic genes (such as α-actin, IFN-β and IFN-γ) as well as regulate some viral promoters (1). YY1 deficient embryos die approximately at the time of implantation, suggesting that YY1 has an essential role in embryonic development (2). YY1 is overexpressed in cancer cellssuch as prostate cancer and therfore may be considered a prognostic marker (1).

  1. Gordon, S. et al. (2006) Oncogene 25, 1125-42.
  2. Donohoe, M.E. et al. (1999) Mol. Cell Biol. 19, 7237-7244.

Application References

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Companion Products

Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.

For Research Use Only. Not For Use In Diagnostic Procedures.

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