Product Pathways - Neuroscience
Presenilin 2 Antibody #2192
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP IHC-P IF-IC | H M R Mk | Endogenous | 23 (C-terminal fragment). 54 (full-length). | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Presenilin 2 Antibody detects endogenous levels of the carboxy-terminal fragment of presenilin 2 (~20 kDa) and the full length protein (54 kDa) to a lesser extent. It does not cross-react with endogenous levels of presenilin 1.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues surrounding amino acid 330 of human presenilin 2. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from human (293 and HeLa), mouse (NIH/3T3 and L929) and rat (C6 and PC12) cell lines, using Presenilin 2 Antibody.
IHC-P (paraffin)
Immunohistochemical staining of paraffin-embedded human brain, showing nuclear and cytoplasmic localization, using Presenilin 2 Antibody.
IF-IC
Confocal microscopic images of PC-12 cells showing ER and nuclear labeling with Presenilin 2 Antibody (A) compared to an isotype control (B). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).
Background
Presenilin 1 and presenilin 2 are transmembrane proteins belonging to the presenilin family. Mutation of presenilin genes has been linked to early onset of Alzheimer's disease, probably due to presenilin's associated gamma-secretase activity for amyloid-beta protein processing (1,2). Endogenous presenilin mainly exists in a heterodimeric complex formed from the endoproteolytically processed amino-terminal (34 kDa) and carboxy-terminal (~20, 22, 23 kDa) fragments (2,3).
- Haass, C. and DeStrooper, B. (1999) Science 286, 916-919.
- Kimberly, W.T. et al. (2000) J. Biol. Chem. 275, 3173-3178.
- Kim, T.W. et al. (1997) J. Biol. Chem. 272, 11006-11010.
Application References
- Hashimoto, Y. et al. (2002) Neurotoxic mechanisms by Alzheimer's disease-linked N141I mutant presenilin 2. J. Pharmacol. Exp. Ther. 300 (3), 736-745. This article references the use of Presenilin 2 Antibody in the following applications: Western Blotting
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
