Product Pathways - Neuroscience
Presenilin 2 Antibody #2192
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP IHC-P | H M R Mk | Endogenous | 23 (C-terminal fragment). 54 (full-length). | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by Western blot.
Protocols
Specificity / Sensitivity
Presenilin 2 Antibody detects endogenous levels of the carboxy-terminal fragment of presenilin 2 (~20 kDa) and the full length protein (54 kDa) to a lesser extent. It does not cross-react with endogenous levels of presenilin 1.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding amino acid 330 of human presenilin 2. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from human (293 and HeLa), mouse (NIH/3T3 and L929) and rat (C6 and PC12) cell lines, using Presenilin 2 Antibody.
IHC-P (paraffin)
Immunohistochemical staining of paraffin-embedded human brain, showing nuclear and cytoplasmic localization, using Presenilin 2 Antibody.
Background
Presenilin 1 and presenilin 2 are transmembrane proteins belonging to the presenilin family. Mutation of presenilin genes has been linked to early onset of Alzheimer's disease, probably due to presenilin's associated gamma-secretase activity for amyloid-beta protein processing (1,2). Endogenous presenilin mainly exists in a heterodimeric complex formed from the endoproteolytically processed amino-terminal (34 kDa) and carboxy-terminal (~20, 22, 23 kDa) fragments (2,3).
- Haass, C. and DeStrooper, B. (1999) Science 286, 916-919.
- Kimberly, W.T. et al. (2000) J. Biol. Chem. 275, 3173-3178.
- Kim, T.W. et al. (1997) J. Biol. Chem. 272, 11006-11010.
Application References
- Hashimoto, Y. et al. (2002) Neurotoxic mechanisms by Alzheimer's disease-linked N141I mutant presenilin 2. J. Pharmacol. Exp. Ther. 300 (3), 736-745. Applications: Western Blotting
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
