Cell Signaling Technology

Product Pathways - Protein Stability

MG-132 #2194

Molecular Formula:
C26H41N3O5
Molecular Weight:
475.6 g/mol
Purity:
>98%

Directions for Use

MG-132 is supplied as a lyophilized powder. For a 10 mM stock, reconstitute the 1 mg in 210.3 µl DMSO. Working concentrations and length of treatments vary depending on the desired effect, but it is typically used at 5-50 µM for 1-24 hours. Soluble in DMSO or EtOH.

Western Blotting

Western Blotting

Western blot analysis of extracts from HT-1080 cells, untreated (-) or treated with MG-132 (10 μM, 4 hr; +), using Phospho-Cyclin D1 (Thr286) (D29B3) XP® Rabbit mAb #3300.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 and HeLa cells, untreated (-) or treated with MG-132 (50 µM, 90 min; +), using Ubiquitin (P4D1) Mouse mAb #3936.

Structure

Structure

Chemical structure of MG-132.


Background

MG-132 is a potent proteasome and calpain inhibitor. Research studies have shown that MG-132 prevents degradation of the proteasome substrates SucLLVY-MCA and ZLLL-MCA with an IC50 of 850 nM and 100 nM, respectively, as well as inhibits casein-degrading activity of m-calpain with an IC50 of 1.25 µM (1). Investigators have demonstrated that MG-132 inhibits TNF-α-induced NF-κB activation and IL-8 release in A549 cells (2). Proteasome inhibition with MG-132 prevents degradation of short-lived proteins, which correlates with increased expression of HSP and ER chaperone proteins (3). MG-132 induces apoptosis in Hep G2 cells in a time- and dose-dependent manner (4). Proteasome inhibitors like MG-132 are important research tools for studying cellular degradation of the ubiquitin-proteasome pathway.

  1. Tsubuki, S. et al. (1996) J Biochem 119, 572-6.
  2. Fiedler, M.A. et al. (1998) Am J Respir Cell Mol Biol 19, 259-68.
  3. Bush, K.T. et al. (1997) J Biol Chem 272, 9086-92.
  4. Emanuele, S. et al. (2002) Int J Oncol 21, 857-65.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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