Cell Signaling Technology

Product Pathways - Translational Control

Mnk1 (C4C1) Rabbit mAb #2195

Applications Reactivity Sensitivity MW (kDa) Isotype
W H M Endogenous 50 Rabbit IgG

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse
Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Mnk1 (C4C1) Rabbit mAb detects endogenous levels of total Mnk1 protein.

Source / Purification

Mnk1 (C4C1) Rabbit mAb is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) derived from the sequence of human Mnk1.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Mnk1 (C4C1) Rabbit mAb.

Background

Mitogen-activated protein kinases (MAPKs) are activated by various extracellular signals and play crucial roles in regulating cell proliferation, differentiation, survival, and apoptosis (1). MAPK-interacting kinases (Mnks or MKNKs) are direct downstream substrates of MAPK and were first discovered independently by the work of Fukunaga and Hunter (2) and Waskiewicz and Cooper (3). There are 2 Mnks in human, termed Mnk1 and Mnk2. Both Mnks possess a MAPK-binding domain that allows them to bind to and then to be phosphorylated by Erk and p38. The phosphorylation in the T-loop of Mnks stimulates their in vitro kinase activity toward a substrate, eukaryotic initiation factor-4E (eIF4E) (2,3). eIF4E is a key component of the translational machinery mediating the initiation of translation, but how phosphorylation of eIF4E regulates translation initiation is still under investigation (4).

  1. Chang, L. and Karin, M. (2001) Nature 410, 37-40.
  2. Fukunaga, R. and Hunter, T. (1997) EMBO J. 16, 1921-1933.
  3. Waskiewicz, A.J. et al. (1997) EMBO J. 16, 1909-1920.
  4. Scheper, G.C. and Proud, C.G. (2002) Eur. J. Biochem. 269, 5350-5359.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

Products