Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

ESET (C1C12) Rabbit mAb #2196

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IF-IC H Mk Endogenous 180 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

ESET (C1C12) Rabbit mAb detects endogenous levels of total ESET protein. The antibody does not cross-react with other SET-domain containing histone methyltransferase proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of the human ESET protein.

Western Blotting

Western Blotting

Western blot analysis of cell lysates from MCF7 and 293 cells using ESET (C1C12) Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of MCF-7 cells using ESET (C1C12) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).

Background

The Erg-associated protein with SET domain (ESET), also known as SET-domain, bifurcated 1 (SETDB1) protein, is a member of a family of histone lysine methyltransferases, each of which contains a conserved catalytic SET domain originally identified in Drosophila Su[var]3-9, Enhancer of zeste, and Trithorax proteins (1). ESET also contains tudor and methyl-CpG-binding domains, which may coordinate binding to methylated histones and methylated DNA, respectively (1). ESET methylates histone H3 Lys9, creating a transcriptionally repressive mark that facilitates gene silencing (1-3). However, unlike SUV39H histone H3 Lys9 methyltransferases, which function mainly in heterochromatin regions such as pericentric heterochromatin, ESET functions mainly in euchromatic regions to repress gene promoters (3). ESET interacts with a variety of proteins, including transcription factors (ERG), histone deacetylases (HDAC1/2), DNA methyltransferases (DNMT3A/B) and transcriptional co-repressors (mSin3A/B, MBD1, KAP-1, the ATFa-associated modulator mAM) (1-6). mAM forms a complex with ESET, stimulating its methyltransferase activity, specifically the conversion of di-methyl to tri-methyl histone H3 Lys9 (2). MBD1 recruits ESET to the CAF-1 complex to facilitate methylation of histone H3 Lys9 during replication-coupled chromatin assembly in S phase (5). DNMT3A recruits ESET to silenced promoters in cancer cells (7). ESET may play a role in the pathogenesis of Huntington's disease, since levels of ESET protein and tri-methyl histone H3 Lys9 are both increased in diseased brains (8).

  1. Yang, L. et al. (2002) Oncogene 21, 148-152.
  2. Wang, H. et al. (2003) Mol. Cell 12, 475-487.
  3. Schultz, D.C. et al. (2002) Genes Dev. 16, 919-932.
  4. Yang, L. et al. (2003) Biochem. J. 369, 651-657.
  5. Sarraf, S.A. and Stancheva, I. (2004) Mol. Cell 15, 595-605.
  6. Ichimura, T. et al. (2005) J. Biol. Chem. 280, 13928-13935.
  7. Li, H. et al. (2006) J. Biol. Chem. 281, 19489-19500.
  8. Ryu, H. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 19176-19181.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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