Product Pathways - Chromatin Regulation / Epigenetics
ESET (C1C12) Rabbit mAb #2196
|2196S||100 µl (10 western blots)||---||In Stock||---|
|2196||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Monkey||Endogenous||180||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
ESET (C1C12) Rabbit mAb detects endogenous levels of total ESET protein. The antibody does not cross-react with other SET-domain containing histone methyltransferase proteins.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of the human ESET protein.
Western blot analysis of cell lysates from MCF7 and 293 cells using ESET (C1C12) Rabbit mAb.
The Erg-associated protein with SET domain (ESET), also known as SET-domain, bifurcated 1 (SETDB1) protein, is a member of a family of histone lysine methyltransferases, each of which contains a conserved catalytic SET domain originally identified in Drosophila Su[var]3-9, Enhancer of zeste, and Trithorax proteins (1). ESET also contains tudor and methyl-CpG-binding domains, which may coordinate binding to methylated histones and methylated DNA, respectively (1). ESET methylates histone H3 Lys9, creating a transcriptionally repressive mark that facilitates gene silencing (1-3). However, unlike SUV39H histone H3 Lys9 methyltransferases, which function mainly in heterochromatin regions such as pericentric heterochromatin, ESET functions mainly in euchromatic regions to repress gene promoters (3). ESET interacts with a variety of proteins, including transcription factors (ERG), histone deacetylases (HDAC1/2), DNA methyltransferases (DNMT3A/B) and transcriptional co-repressors (mSin3A/B, MBD1, KAP-1, the ATFa-associated modulator mAM) (1-6). mAM forms a complex with ESET, stimulating its methyltransferase activity, specifically the conversion of di-methyl to tri-methyl histone H3 Lys9 (2). MBD1 recruits ESET to the CAF-1 complex to facilitate methylation of histone H3 Lys9 during replication-coupled chromatin assembly in S phase (5). DNMT3A recruits ESET to silenced promoters in cancer cells (7). ESET may play a role in the pathogenesis of Huntington's disease, since levels of ESET protein and tri-methyl histone H3 Lys9 are both increased in diseased brains (8).
- Yang, L. et al. (2002) Oncogene 21, 148-152.
- Wang, H. et al. (2003) Mol. Cell 12, 475-487.
- Schultz, D.C. et al. (2002) Genes Dev. 16, 919-932.
- Yang, L. et al. (2003) Biochem. J. 369, 651-657.
- Sarraf, S.A. and Stancheva, I. (2004) Mol. Cell 15, 595-605.
- Ichimura, T. et al. (2005) J. Biol. Chem. 280, 13928-13935.
- Li, H. et al. (2006) J. Biol. Chem. 281, 19489-19500.
- Ryu, H. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 19176-19181.
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For Research Use Only. Not For Use In Diagnostic Procedures.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
This antibody is developed, validated, and produced by CST using in part technology under license (granting certain rights including those under U.S. Patent No. 5,675,063) from Epitomics, Inc.