Cell Signaling Technology

Product Pathways - Apoptosis

Etoposide #2200

Molecular Formula:
C29H32O13
Molecular Weight:
588.56 g/mol
Purity:
>99%

Directions for Use

Etoposide is supplied as a lyophilized powder. For a 50 mM stock, reconstitute the 5.9 mg in 200 µl DMSO. Working concentrations and length of treatments vary depending on the desired effect, but it is typically used at 5-50 µM for 4-24 hr. Soluble in DMSO at 100 mg/ml; very poorly soluble in ethanol and water with maximum solubility in water ~20-50 µM.

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat cells, untreated (-) or etoposide-treated (25 μM, overnight; +), using Cleaved PARP (Asp214) (D64E10) XP® Rabbit mAb #5625 (upper) or total PARP Antibody #9542 (lower).

Structure

Structure

Chemical structure of etoposide.

Background

An anti-tumor agent that is commonly used as an apoptosis inducer, etoposide (VP-16) is a topoisomerase II inhibitor with an IC50 of 59.2 µM (1). Etoposide stabilizes a covalent topoisomerase II-cleaved DNA intermediate complex in the catalytic cycle of the enzyme, leading to genomic instability and cell death (2,3). This mechanism of action has been shown to delay progression of the cell cycle through the late S and early G2 phase (4,5).

  1. Terada, T. et al. (1993) J Med Chem 36, 1689-99.
  2. Baldwin, E.L. and Osheroff, N. (2005) Curr Med Chem Anticancer Agents 5, 363-72.
  3. Li, T.K. and Liu, L.F. (2001) Annu Rev Pharmacol Toxicol 41, 53-77.
  4. Dołega, A. (1998) Postepy Hig Med Dosw 52, 67-87.
  5. Smith, P.J. et al. (1994) Br J Cancer 70, 914-21.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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