Cell Signaling Technology

Product Pathways - Development

Shh (C9C5) Rabbit mAb #2207

Applications Reactivity Sensitivity MW (kDa) Isotype
W H R Z (M) Endogenous 19, (45 kDa precursor) Rabbit IgG

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Z=Zebrafish
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Shh (C9C5) Rabbit mAb detects the endogenous levels of total Shh protein. This antibody does not cross-react with transfected IHH and DHH.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu53 of human Shh.

Western Blotting

Western Blotting

Western blot analysis of extracts from HT29 and GH3 cells, and GH3 cell conditioned medium (CM), using Shh (C9C5) Rabbit mAb.

Background

Hedgehog proteins (Hh) are secreted signaling proteins that play many roles during animal development. Aberrant Hh signaling activity can be associated with numerous birth defects and uncontrolled Hh pathway activation is linked to the development of several types of cancers (1-2). The three identified vertebrate Hh genes are Sonic (Shh), Indian (Ihh) and Desert (Dhh), all of which have distinct as well as overlapping roles (3-5). Hh proteins are synthesized as 45 kDa precursors that undergo auto-cleavage to generate a 19 kDa amino-terminal peptide (Hh-N) and a carboxy-terminal peptide (Hh-C). The amino-terminal peptide becomes covalently attached to a cholesterol molecule at its carboxy terminus and acetylated at its amino terminus. This doubly modified Hh-N peptide is released from cells and responsible for all known Hedgehog signaling activity (6).

  1. Ingham, P.W. and McMahon, A.P. (2001) Genes Dev. 15, 3059-3087.
  2. McMahon, A.P. et al. (2003) Curr. Top Dev. Biol. 53, 1-114.
  3. Zhang, X.M. et al. (2001) Cell 106, 781-792.
  4. Adolphe, C. et al. (2004) Development 131, 5009-5019.
  5. Pathi, S. et al. (2001) Mech. Dev. 106, 107-117.
  6. Bijlsma, M.F. et al. (2004) Bioessays 26, 387-394.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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