Product Pathways - Glucose Metabolism
Glut4 (1F8) Mouse mAb #2213
PhosphoSitePlus® protein, site, and accession data: GLUT4
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W | H M R | Endogenous | 50 | Mouse IgG1 |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 2213:
- Western Blotting
Specificity / Sensitivity
Glut4 (1F8) Mouse mAb detects endogenous levels of total Glut4 protein. It does not cross-react with other related proteins.
Source / Purification
Monoclonal antibody is produced by immunizing animals with partially purified intracellular glucose transporter-containing vesicles obtained from rat adipocytes.
Western Blotting
Western blot analysis of extracts from NIH/3T3 cells using Glut4 (1F8) Mouse mAb.
Background
A group of related glucose transporters (Glut1-5 and 7) mediate the facilitated diffusion of glucose in nonepithelial mammalian tissues. Within insulin-responsive tissues such as muscle and fat, Glut1 contributes to basal glucose uptake while Glut4 is responsible for insulin-stimulated glucose transport (1-3). Glut4 is a 12-transmembrane domain protein that facilitates glucose transport in the direction of the glucose gradient. This transporter localizes to intracellular organelles (endosomes) in unstimulated cells and translocates to the cell surface following insulin stimulation (1,2,4). Translocation of Glut4 is dependent on Akt, which may act by phosphorylating AS160, a RabGAP protein involved in membrane trafficking (5).
- Marette, A. et al. (1992) Am. J. Physiol. 263, C443-C452.
- Slot, J.W. et al. (1991) Proc. Natl. Acad. Sci. USA 88, 7815-7819.
- James, D.E. et al. (1989) Nature 338, 83-87.
- Barrett, M.P. et al. (1999) Curr. Opin. Cell Biol. 11, 496-502.
- Zeigerer, A. et al. (2004) Mol. Biol. Cell 15, 4406-4415.
Application References
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
