Product Pathways - Translational Control

Phospho-S6 Ribosomal Protein (Ser240/244) Antibody #2215

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PhosphoSitePlus^® protein, site, and accession data: S6

Applications	Reactivity	Sensitivity	MW (kDa)	Source
W IP	H M R Mk Z (C) (X)	Endogenous	32	Rabbit

Applications Key: W=Western Blotting IP=Immunoprecipitation
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey C=Chicken X=Xenopus Z=Zebrafish
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

2215:: Immunoprecipitation, Western Blotting

Specificity / Sensitivity

Phospho-S6 Ribosomal Protein (Ser240/244) Antibody detects endogenous levels of ribosomal protein S6 only when phosphorylated at serines 240 and 244. This antibody does not detect S6 ribosomal protein phosphorylated at other sites.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser240 and Ser244 of human S6 ribosomal protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western blot analysis of extracts from 293 cells, untreated or treated with 20% FBS for the indicated time, using Phospho-S6 Ribosomal Protein (Ser235/236) Antibody #2211 (upper) and Phospho-S6 Ribosomal Protein (Ser240/244) Antibody #2215 (lower).

Background

One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of the S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5' untranslated regions (2). These particular mRNA transcripts (5'TOP) encode proteins involved in cell cycle progression as well as ribosomal proteins and elongation factors necessary for translation (2,3). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of the S6 protein (4,5).

Application References

Boylan, J. et al. (2001) Ribosomal protein S6 phosphorylation and function during late gestation liver development in the rat. J. Biol. Chem. 276, 44457-44463. Applications: Western Blotting
Tang, H. et al. (2001) Amino acid-induced translation of TOP mRNAs is fully dependent on phosphatidylinositol 3-kinase-mediated signaling, is partially inhibited by rapamycin, and is independent of S6K1 and rpS6 phosphorylation. Mol. Cell. Biol. 21, 8671-8683. Applications: Western Blotting
Cao, R. et al. (2008) Mol Cell Neurosci 38, 312-24. Applications: IF-IC (In Cells)
Fonseca, B.D. et al. (2011) J Biol Chem 286, 27111-22. Applications: Western Blotting
Goto, J. et al. (2011) Proc Natl Acad Sci U S A 108, E1070-9. Applications: Western Blotting

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.