Product Pathways -
Myc-Tag (9B11) Mouse mAb (Alexa Fluor® 647 Conjugate) #2233
| Applications | Reactivity | Sensitivity | Isotype |
|---|---|---|---|
| IF-IC F | All | Transfected Only | Mouse IgG2a |
Applications Key:
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key: All=All species expected
Species cross-reactivity is determined by western blot.
Protocols
- 2233:
- Flow, Immunofluorescence
Specificity / Sensitivity
Myc-Tag (9B11) Mouse mAb (Alexa Fluor® 647 Conjugate) detects transfected proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag fused to either the amino or carboxy terminus of targeted proteins in transfected mammalian cells.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues 410-419 of human c-Myc (EQKLISEEDL). The antibody was conjugated to Alexa Fluor® 647 under optimal conditions with an F/P ratio of 2-6. The Alexa Fluor® 647 dye is maximally excited by red light (e.g. 633 nm He-Ne laser). Antibody conjugates of the Alexa Fluor® 647 dye produce bright far-red-fluorescence emission, with a peak at 665 nm.
Flow Cytometry
Flow cytometric analysis of COS cells, untransfected (blue) or transfected with Myc-tagged Akt (green), using Myc-Tag (9B11) Mouse mAb (Alexa Fluor® 647 Conjugate).
IF-IC
Confocal immunofluorescent analysis of COS cells expressing a Myc-tagged protein (left) and untreated (right), using Myc-Tag (9B11) Mouse mAb (Alexa Fluor® 647 Conjugate) (blue pseudocolor). Actin filaments have been labeled with DY-554 phalloidin (red).
Description
This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in cells transfected with Myc-tagged protein.
Background
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.
The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 2279 Myc-Tag (9B11) Mouse mAb (Alexa Fluor® 488 Conjugate)
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- 2365 His-Tag Antibody
- 2272 Myc-Tag Antibody
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- 2396 MBP (8G1) Mouse mAb
Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
For Research Use Only. Not For Use In Diagnostic Procedures.
