Product Pathways - Tyrosine Kinase/ Adaptors
Phospho-EGF Receptor (Tyr992) Antibody #2235
| Applications | Reactivity | MW (kDa) | Source |
|---|---|---|---|
| W IHC-P | H (M) (R) | 175 | Rabbit |
Applications Key:
W=Western Blotting
IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Phospho-EGF Receptor (Tyr992) Antibody detects endogenous EGF receptor only when phosphorylated at tyrosine 992. The antibody may cross-react with other activated EGF receptor family members (e.g. ErbB2).
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Tyr992 of human EGF receptor. Antibodies are purified by protein A and peptide affinity chromatography.
Background
The epidermal growth factor (EGF) receptor is a 170 kDa transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Ligand binding results in receptor dimerization, autophosphorylation, activation of downstream signaling and lysosomal degradation (1,2). Phosphorylation of EGF receptor (EGFR) at Tyr845 in the kinase domain is implicated in stabilizing the activation loop, maintaining the active state enzyme and providing a binding surface for substrate proteins (3,4). c-Src is involved in phosphorylation of EGFR at Tyr845 (5). The SH2 domain of PLCγ binds at phospho-Tyr992, resulting in activation of PLCγ-mediated downstream signaling (6). Phosphorylation of EGFR at Tyr1045 creates a major docking site for c-Cbl, an adaptor protein that leads to receptor ubiquitination and degradation following EGFR activation (7,8). The GRB2 adaptor protein binds activated EGFR at phospho-Tyr1068 (9). A pair of phosphorylated residues (Tyr1148 and Tyr1173) provides a docking site for the SHC scaffold protein, with both sites involved in MAP kinase signaling activation (2). Phosphorylation of EGFR at specific serine and threonine residues attenuates EGFR kinase activity. EGFR carboxy-terminal residues Ser1046 and Ser1047 are phosphorylated by CaM kinase II; mutations to either of these serines results in upregulated EGFR tyrosine autokinase activity (10).
- Hackel, P.O. et al. (1999) Curr. Opin. Cell Biol. 11, 184-189.
- Zwick, E. et al. (1999) Trends Pharmacol. Sci. 20, 408-412.
- Cooper, J.A. and Howell, B. (1993) Cell 73, 1051-1054.
- Hubbard, S.R. et al. (1994) Nature 372, 746-754.
- Biscardi, J.S. et al. (1999) J. Biol. Chem. 274, 8335-8343.
- Emlet, D.R. et al. (1997) J. Biol. Chem. 272, 4079-4086.
- Levkowitz, G. et al. (1999) Mol. Cell 4, 1029-1040.
- Ettenberg, S.A. et al. (1999) Oncogene 18, 1855-1866.
- Rojas, M. et al. (1996) J. Biol. Chem. 271, 27456-27461.
- Feinmesser, R.L. et al. (1999) J. Biol. Chem. 274, 16168-16173.
Application References
- Iordanov, M. S. et al. (2002) The UV (ribotoxic) stress response of human keratinocytes involves the unexpected uncoupling of the Ras-extracellular signal-regulated kinase signaling cascade from the activated epidermal growth factor receptor. Mol. Cell. Biol. 22, 5380-5394. This article references the use of Phospho-EGF Receptor (Tyr992) Antibody in the following applications: Western Blotting
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Companion Products
- 2232 EGF Receptor Antibody
- 2234 Phospho-EGF Receptor (Tyr1068) Antibody
- 2237 Phospho-EGF Receptor (Tyr1045) Antibody
- 2241 Phospho-HER2/ErbB2 (Tyr877) Antibody
- 2242 HER2/ErbB2 Antibody
- 2244 Phospho-HER2/ErbB2 (Tyr1248)/EGFR (Tyr1173) Antibody
- 9411 Phospho-Tyrosine Mouse mAb (P-Tyr-100)
- 9922 Phospho-EGF Receptor Antibody Sampler Kit
- 9923 Phospho-HER2/ErbB2 Antibody Sampler Kit
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 8102 SignalSlide™ Phospho-EGF Receptor (Tyr1173) IHC Controls