Product Pathways - Tyrosine Kinase / Adaptors
HER2/ErbB2 (44E7) Mouse mAb #2248
|W||H (M) (R)||Endogenous||185||Mouse IgG1|
Reactivity Key: H=Human M=Mouse R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
HER2/ErbB2 (44E7) Mouse mAb detects endogenous levels of Her2/ErbB2 in various cell lines. It does not cross-react with any other related proteins.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues close to the carboxy-terminal sequence of human Her2/ErbB2.
Western blot analysis of various cell lysates using HER2/ErbB2 (44E7) Mouse mAb. HER2/ErbB2 (44E7) Mouse mAb specifically recognizes Her2/ErbB2 in SK-BR-3, MDA-MB-453 and T47D cells, but does not cross-react with EGF receptors (ovexpressed in A431 cells), ErbB3 (overexpressed in Cos-7 cells), or ErbB4 (overexpressed in CEM cells). ( The CEM-ErbB4 cell lysate was provided by Dr. David Riese, Purdue University).
The ErbB2 (HER2) proto-oncogene encodes a 185 kDa transmembrane, receptor-like glycoprotein with intrinsic tyrosine kinase activity (1). While ErbB2 lacks an identified ligand, ErbB2 kinase activity can be activated in the absence of a ligand when overexpressed and through heteromeric associations with other ErbB family members (2). Amplification of the ErbB2 gene and overexpression of its product are detected in almost 40% of human breast cancers (3). Binding of the c-Cbl ubiquitin ligase to ErbB2 at Tyr1112 leads to ErbB2 poly-ubiquitination and enhances degradation of this kinase (4). ErbB2 is a key therapeutic target in the treatment of breast cancer and other carcinomas and targeting the regulation of ErbB2 degradation by the c-Cbl-regulated proteolytic pathway is one potential therapeutic strategy. Phosphorylation of the kinase domain residue Tyr877 of ErbB2 (homologous to Tyr416 of pp60c-Src) may be involved in regulating ErbB2 biological activity. The major autophosphorylation sites in ErbB2 are Tyr1248 and Tyr1221/1222; phosphorylation of these sites couples ErbB2 to the Ras-Raf-MAP kinase signal transduction pathway (1,5).
- Muthuswamy, S.K. et al. (1999) Mol Cell Biol 19, 6845-57.
- Qian, X. et al. (1994) Proc Natl Acad Sci USA 91, 1500-4.
- Dittadi, R. and Gion, M. (2000) J Natl Cancer Inst 92, 1443-4.
- Klapper, L.N. et al. (2000) Cancer Res 60, 3384-8.
- Kwon, Y.K. et al. (1997) J Neurosci 17, 8293-9.
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- 9923 Phospho-HER2/ErbB2 Antibody Sampler Kit
- 2243 Phospho-HER2/ErbB2 (Tyr1221/1222) (6B12) Rabbit mAb
- 2165 HER2/ErbB2 (29D8) Rabbit mAb
- 2241 Phospho-HER2/ErbB2 (Tyr877) Antibody
- 2242 HER2/ErbB2 Antibody
- 2244 Phospho-HER2/ErbB2 (Tyr1248)/EGFR (Tyr1173) Antibody
- 2247 Phospho-HER2/ErbB2 (Tyr1248) Antibody
- 2249 Phospho-HER2/ErbB2 (Tyr1221/1222) Antibody
- 3250 HER2/ErbB2 (M45) Antibody
- 7072 Phototope®-HRP Western Blot Detection System, Anti-mouse IgG, HRP-linked Antibody
- 7076 Anti-mouse IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.