Product Pathways - Lymphocyte Signaling
PU.1 (9G7) Rabbit mAb #2258
| Applications | Reactivity | MW (kDa) | Source | Isotype |
|---|---|---|---|---|
| W IP IHC-P IF-IC F | H M (Mk) | 42 | Rabbit | IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
This antibody detects endogenous levels of total PU.1 protein. The antibody does not cross react with other Ets family members.
Source / Purification
Monoclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) derived from the sequence of human PU.1 protein.
Western Blotting
Western blot analysis of extracts from RAW, THP1 and p388D1 cells, using PU.1 (9G7) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded 4T1 syngeneic mouse tumor, using PU.1 (9G7) Rabbit mAb #2258.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded Non-Hodgkin's lymphoma, using PU.1 (9G7) Rabbit mAb in the presence of control peptide (left) or PU.1 Blocking Peptide #1036 (right).
Flow Cytometry
Flow cytometric analysis of untreated THP-1 cells, using PU.1 (9G7) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).
IF-IC
DAPI staining (left) and immunofluorescent staining (right) of paraformaldehyde-fixed RAW cells, using PU.1 (9G7) Rabbit mAb.
Background
PU.1 is a member of the Ets family of transcription factors and activates target genes through the purine-rich PU-box (1). PU.1 plays a pivotal role in the differentiation of myeloid cells and lymphocytes and is expressed in several hematopoietic cells including B lymphocytes, macrophages, neutrophils, mast cells, early erythroid cells and megakaryocytes (1,2). The concentration of PU.1 is critical for both the determination of hematopoietic cell lineage and the regulation of differentiation versus stem cell proliferation (3,4). In addition, PU.1 activity is influenced by phosphorylation and interactions with other hematopoietic transcription factors. Phosphorylation of PU.1 at Ser146 by CK2 promotes binding to IRF4 and synergistic activation through the immunoglobulin κ 3' enhancer (5). Treatment of pro-B cells with IL-3 leads to phosphorylation of PU.1 at Ser140, resulting in increased PU.1 activity and activation of the anti-apoptotic gene MCL-1 (6). GATA1 binding blocks PU.1 activity during erythroid cell development (7). Overexpression of PU.1 resulting from proviral insertion during Friend virus infection can induce erythroleukemia, while reduced expression has been associated with acute myeloid leukemia (8).
- Lloberas, J. et al. (1999) Immunol. Today 20, 184-189.
- Klemsz, M.J. et al. (1990) Cell 61, 113-124.
- Dahl, R. and Simon, M.C. (2003) Blood Cells Mol. Dis. 31, 229-233.
- DeKoter, R.P. and Singh, H. (2000) Science 288, 1439-1441.
- Pongubala, J.M. et al. (1993) Science 259, 1622-1625.
- Wang, J.M. et al. (2003) Mol. Cell Biol. 23, 1896-1909.
- Zhang, P. et al. (1999) Proc. Natl. Acad. Sci. USA 96, 8705-8710.
- Moreau-Gachelin, F. et al. (1998) Nature 331, 277-280.
Application References
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Companion Products
- 2266 PU.1 Antibody
- 4964 IRF-4 Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 1036 PU.1 Blocking Peptide
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
