Product Pathways - Phosphatases

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PP2A C Subunit (52F8) Rabbit mAb #2259

Applications	Reactivity	Sensitivity	MW (kDa)	Isotype
W IP IHC-P F	H M R Mk Dm (C) (Pg)	Endogenous	36, 38	Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  C=Chicken  Dm=D. melanogaster  Pg=Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

2259:

Flow, IHC / Paraffin, Immunoprecipitation, Western Blotting

Specificity / Sensitivity

PP2A C Subunit (52F8D8) Antibody detects endogenous levels of PP2A catalytic subunit protein (both alpha and beta isoforms). The antibody does not cross-react with other PP2A subunits.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human PP2A C subunit protein.

Background

Protein phosphatase type 2A (PP2A) is an essential protein serine/threonine phosphatase that is conserved in all eukaryotes. PP2A is a key enzyme within various signal transduction pathways as it regulates fundamental cellular activities such as DNA replication, transcription, translation, metabolism, cell cycle progression, cell division, apoptosis and development (1-3). The core enzyme consists of catalytic C and regulatory A (or PR65) subunits, with each subunit represented by α and β isoforms (1). Additional regulatory subunits belong to four different families of unrelated proteins. Both the B (or PR55) and B' regulatory protein families contain α, β, γ and δ isoforms, with the B' family also including an ε protein. B'' family proteins include PR72, PR130, PR59 and PR48 isoforms, while striatin (PR110) and SG2NA (PR93) are both members of the B''' regulatory protein family. These B subunits competitively bind to a shared binding site on the core A subunit (1). This variable array of holoenzyme components, particularly regulatory B subunits, allows PP2A to act in a diverse set of functions. PP2A function is regulated by expression, localization, holoenzyme composition and post-translational modification. Phosphorylation of PP2A at Tyr307 by Src occurs in response to EGF or insulin and results in a substantial reduction of PP2A activity (4). Reversible methylation on the carboxyl group of Leu309 of PP2A has been observed (5,6). Methylation alters the conformation of PP2A, as well as its localization and association with B regulatory subunits (6-8).

1. Janssens, V. and Goris, J. (2001) Biochem. J. 353, 417-439.
2. Zolnierowicz, S. (2000) Biochem. Pharmacol. 60, 1225-1235.
3. Milward, T.A. et al. (1999) Trends Biochem. Sci. 24, 186-191.
4. Chen, J. et al. (1992) Science 257, 1261-1264.
5. Turowski, P. et al. (1995) J. Cell. Biol. 129, 397-410.
6. Lee, J. et al. (1996) Proc. Natl. Acad. Sci. USA 93, 6043-6047.
7. Tolstykh, T. et al. (2000) EMBO J. 19, 5682-5691.
8. Yu, X.X. et al. (2001) Mol. Biol. Cell 12, 185-199.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

• 2038 PP2A C Subunit Antibody
• 2039 PP2A A Subunit Antibody
• 4953 PP2A B Subunit Antibody
• 7071 Phototope^®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide
• 1067 PP2A C Subunit Blocking Peptide
• 8112 SignalStain^® Antibody Diluent

Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.

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For Research Use Only. Not For Use In Diagnostic Procedures.