Cell Signaling Technology

Product Pathways - Wnt / Hedgehog / Notch

Naked1 Antibody #2262

Applications Reactivity MW (kDa) Source
W IP H M 59, 61 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Naked1 antibody detects endogenous levels of total Naked1 proteins.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues surrounding Gln227 of human Naked1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of total cell extracts of SW620 and HepG2 cells, using Naked1 Antibody.

Background

Naked1 (Nkd1) and Naked2 (Nkd2) are homologs of Drosophila Naked cuticle, a negative regulator of Wnt/Wingless signaling pathway which functions through a feedback mechanism (1,2). Both Drosophila and vertebrate Naked proteins contain a putative calcium-binding EF-hand motif; however, Drosophila Naked binds to zinc instead of calcium (3). Naked inhibits the canonical Wnt/β-catenin pathway by binding to Dishevelled proteins and directs Dishevelled activity towards the planar cell polarity pathway (2,4). Naked1 is a direct target of Wnt signaling and is overexpressed in some colon tumors due to constitutive activation of Wnt/β-catenin pathway (5). Naked2 is myristoylated and is required for sorting of TGF-α to the basolateral plasma membrane of polarized epithelial cells (6).

  1. Zeng, W. et al. (2000) Nature 403, 789-795.
  2. Wharton, K.A. et al. (2001) Dev. Biol. 234, 93-106.
  3. Rousset, R. et al. (2002) J. Biol. Chem. 277, 49019-49026.
  4. Yan, D. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 3802-3807.
  5. Yan, D. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 14973-14978.
  6. Li, C. et al. (2004) Proc. Natl. Acad. Sci. USA 101, 5571-5576.

Application References

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