Product Pathways - Protein Stability
MMP-9 (G657) Antibody #2270
PhosphoSitePlus® protein, site, and accession data: MMP9
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H | Endogenous | 92kDa | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 2270:
- Western Blotting
Specificity / Sensitivity
MMP-9 (G657) Antibody detects full length (proenzyme, 92 kDa) human MMP-9.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to a sequence based around amino acid residue Gly657 of human MMP-9. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of partially purified, concentrated, serum free medium containing human MMP-9, using MMP-9 (G567) Antibody.
Background
The matrix metalloproteinases (MMPs) are a family of proteases that target many extracellular proteins including other proteases, growth factors, cell surface receptors and adhesion molecules (1). Among the family members, MMP-2, MMP-3, MMP-7 and MMP-9 have been characterized as important factors for normal tissue remodeling during embryonic development, wound healing, tumor invasion, angiogenesis, carcinogenesis and apoptosis (2-4). MMP activity correlates with cancer development (2). One mechanism of MMP regulation is transcriptional (5). Once synthesized, MMP exists as a latent proenzyme. Maximum MMP activity requires proteolytic cleavage to generate active MMPs by releasing the inhibitory propeptide domain from the full length protein (5).
- McCawley, L.J. and Matrisian, L.M. (2001) Curr. Opin. Cell Biol. 13, 534-540.
- Coussens, L.M. et al. (2002) Science 295, 2387-2391.
- Sternlicht, M.D. et al. (1999) Cell 98, 137-146.
- Vu, T.H. et al. (1998) Cell 93, 411-422.
- Nagase, H. et al. (1990) Biochemistry 29, 5783-5789.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 3852 MMP-9 Antibody
- 4022 MMP-2 Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
