Cell Signaling Technology

Product Pathways - Wnt / Hedgehog / Notch

Shh/Ihh Antibody #2271

Applications Reactivity Sensitivity MW (kDa) Source
W M (H) (R) Transfected Only 19, (42,45) Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Shh/Ihh antibody detects both the precursor and mature forms of transfected Shh and transfected Ihh. It is also expected to recognize Shh from cat, dog, chicken and zebra fish based on homology.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu71 of human Shh. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of total cell lysates from 293 cells and 293 cells transiently transfected with mouse Shh or Ihh construct, using Shh/Ihh Antibody.

Background

Hedgehog proteins (Hh) are secreted signaling proteins that play many roles during animal development. Aberrant Hh signaling activity can be associated with numerous birth defects and uncontrolled Hh pathway activation is linked to the development of several types of cancers (1-2). The three identified vertebrate Hh genes are Sonic (Shh), Indian (Ihh) and Desert (Dhh), all of which have distinct as well as overlapping roles (3-5). Hh proteins are synthesized as 45 kDa precursors that undergo auto-cleavage to generate a 19 kDa amino-terminal peptide (Hh-N) and a carboxy-terminal peptide (Hh-C). The amino-terminal peptide becomes covalently attached to a cholesterol molecule at its carboxy terminus and acetylated at its amino terminus. This doubly modified Hh-N peptide is released from cells and responsible for all known Hedgehog signaling activity (6).

  1. Ingham, P.W. and McMahon, A.P. (2001) Genes Dev. 15, 3059-3087.
  2. McMahon, A.P. et al. (2003) Curr. Top Dev. Biol. 53, 1-114.
  3. Zhang, X.M. et al. (2001) Cell 106, 781-792.
  4. Adolphe, C. et al. (2004) Development 131, 5009-5019.
  5. Pathi, S. et al. (2001) Mech. Dev. 106, 107-117.
  6. Bijlsma, M.F. et al. (2004) Bioessays 26, 387-394.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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