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Myc-Tag Antibody #2272
| Applications | Reactivity | Sensitivity | Source |
|---|---|---|---|
| W IP IF-IC | All | Transfected Only | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key: All=All species expected
Specificity / Sensitivity
Myc-Tag Antibody detects transfected proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag fused to either the amino- or carboxy-termini of targeted proteins in transfected mammalian cells.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues 410-419 of human c-Myc (EQKLISEEDL). Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of cell extracts alone (lane 1), cells expressing Myc tagged to the carboxy terminus of the protein (lane 2) and Myc tagged to the amino terminus of the protein (lane 3), using Myc-Tag Antibody.
IP
Immunoprecipitation of cell extracts overexpressing carboxy terminal Myc-tagged protein (lane 1), amino terminal Myc-tagged protein (lane 2) or control cell extracts (lane 3), using Myc-Tag Antibody.
IF-IC
Immunocytochemical staining of cells expressing carboxy-terminal Myc-tagged protein (left) or cells without transfected protein (right), using Myc-Tag Antibody.
Background
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties.
The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems (1).
- Munro, S. and Pelham, H.R. (1984) EMBO J. 3, 3087-3093.
Application References
- Zhang, H. et al. (2002) Enzymes of the SUMO modification pathway localize to filaments of the nuclear pore complex. Mol. Cell. Biol. 22, 6498-6508. This article references the use of Myc-Tag Antibody in the following applications: IC-IF
- Lopes, N. et al. (2003) Thrombospondin 2 regulates cell proliferation induced by Rac1 redox-dependent signaling. Mol. Cell. Biol. 23 (15), 5401-5408. This article references the use of Myc-Tag Antibody in the following applications: Western Blotting
- Shimizu, K. et al. (2002) The fifth essential DNA polymerase phi in Saccharomyces cerevisiae is localized to the nucleolus and plays an important role in synthesis of rRNA. Proc. Natl. Acad. Sci. USA 99, 9133-9138. This article references the use of Myc-Tag Antibody in the following applications: IC-IF IP Western Blotting
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
