Product Pathways - Complementary Reagents
Myc-Tag Antibody #2272
| Applications | Reactivity | Sensitivity | Source |
|---|---|---|---|
| W IF-IC F | All | Transfected Only | Rabbit |
Applications Key:
W=Western Blotting
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key: All=All species expected
Species cross-reactivity is determined by western blot.
Protocols
- 2272:
- Flow, Immunofluorescence, Western Blotting
Specificity / Sensitivity
Myc-Tag Antibody detects recombinant proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues 410-419 of human c-Myc (EQKLISEEDL). Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of cell extracts alone (lane 1), cells expressing Myc tagged to the carboxy terminus of the protein (lane 2) and Myc tagged to the amino terminus of the protein (lane 3), using Myc-Tag Antibody.
Flow Cytometry
Flow cytometric analysis of COS cells, untreated (blue) or Myc transfected (green), using Myc-Tag Antibody.
IF-IC
Confocal immunofluorescent analysis of COS cells transfected with a Myc-tagged protein (left) or untransfected (right) using Myc-Tag Antibody. Actin filaments have been labeled with DY-554 phalloidin (red).
Background
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.
The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems (1).
- Munro, S. and Pelham, H.R. (1984) EMBO J. 3, 3087-3093.
Application References
- Zhang, H. et al. (2002) Enzymes of the SUMO modification pathway localize to filaments of the nuclear pore complex. Mol. Cell. Biol. 22, 6498-6508. Applications: IC-IF
- Lopes, N. et al. (2003) Thrombospondin 2 regulates cell proliferation induced by Rac1 redox-dependent signaling. Mol. Cell. Biol. 23 (15), 5401-5408. Applications: Western Blotting
- Shimizu, K. et al. (2002) The fifth essential DNA polymerase phi in Saccharomyces cerevisiae is localized to the nucleolus and plays an important role in synthesis of rRNA. Proc. Natl. Acad. Sci. USA 99, 9133-9138. Applications: IC-IF IP Western Blotting
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
